概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL-32
  • 宿主
    Rabbit
  • 特异性
    ab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each).
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human IL-32 (C terminal).
    (Peptide available as ab39778)

  • 阳性对照
    • Human spleen lysate, mouse EL4 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab37158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human IL-32 peptide (ab39778). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 18544573

靶标

  • 功能
    Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
  • 组织特异性
    Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 32alpha antibody
    • IL 32beta antibody
    • IL 32delta antibody
    • IL 32gamma antibody
    • IL-32 antibody
    • IL32 antibody
    • IL32_HUMAN antibody
    • Interleukin 32 antibody
    • Interleukin 32 small antibody
    • Interleukin 32 theta antibody
    • Interleukin-32 antibody
    • interleukin-32 eta antibody
    • Natural killer cell transcript 4 antibody
    • Natural killer cells protein 4 antibody
    • NK 4 antibody
    • NK4 antibody
    • OTTHUMP00000236040 antibody
    • OTTHUMP00000236041 antibody
    • OTTHUMP00000236042 antibody
    • OTTHUMP00000236043 antibody
    • OTTHUMP00000236044 antibody
    • OTTHUMP00000236045 antibody
    • OTTHUMP00000236046 antibody
    • OTTHUMP00000236047 antibody
    • OTTHUMP00000236048 antibody
    • OTTHUMP00000236049 antibody
    • OTTHUMP00000236050 antibody
    • OTTHUMP00000236051 antibody
    • OTTHUMP00000236052 antibody
    • OTTHUMP00000236053 antibody
    • OTTHUMP00000236054 antibody
    • OTTHUMP00000241545 antibody
    • OTTHUMP00000241602 antibody
    • OTTHUMP00000241603 antibody
    • OTTHUMP00000241604 antibody
    • OTTHUMP00000241605 antibody
    • OTTHUMP00000241606 antibody
    • OTTHUMP00000241607 antibody
    • OTTHUMP00000241608 antibody
    • OTTHUMP00000241609 antibody
    • OTTHUMP00000241610 antibody
    • OTTHUMP00000241611 antibody
    • OTTHUMP00000241612 antibody
    • TAIF antibody
    • TAIFa antibody
    • TAIFb antibody
    • TAIFc antibody
    • TAIFd antibody
    • Tumor necrosis factor alpha-inducing factor antibody
    see all

图片

  • Immunohistochemical analysis of IL32 expression in mouse spleen tissue with ab37158 at 2µg/ml
  • Lane 1 : Anti-IL-32 antibody (ab37158) at 5 µg/ml
    Lane 2 : Anti-IL-32 antibody (ab37158) at 10 µg/ml

    All lanes : Human spleen cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 12,26 kDa
    why is the actual band size different from the predicted?



    Two isoforms of IL32 can be seen
  • Westen blot data for human spleen cell lysates (left lanes) and mouse EL4 cell lysates (right lanes).
  • IHC-P of Human spleen tissue with ab37158 at 10 µg/ml
  • Immunofluorescence of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.

文献

This product has been referenced in:
  • Gomes RS  et al. IL-32? promotes the healing of murine cutaneous lesions caused by Leishmania braziliensis infection in contrast to Leishmania amazonensis. Parasit Vectors 10:336 (2017). IHC-P ; Human . Read more (PubMed: 28709468) »
  • Bi D  et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). Read more (PubMed: 28912850) »
See all 7 Publications for this product

客户评价及客户问答

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human skin squamous cell carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Human skin squamous cell carcinoma
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 2%
Fixative
Paraformaldehyde

Mr. Yong Sun Lee

Verified customer

提交于 Jun 27 2018

Answer

Thank you for your enquiry.

I can confirm that ab37158 was tested under both reducing and denaturing conditions. Here is the standard protocol for sample sample preparation.

Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

We have not tried the antibody under native WB but as it works well in IHC and IF, there is a good chance it could detect the native protein in other assays.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us.

I have attached the filled out Exel spreadsheet with all the requested information. To be fair, all the information asked could be answered with a look on the datasheet...

In addition, I had a look through our tissue slices for her IHC-P experiments. Instead of the Tonsils she can use Lymphknot (as we do not have Tonsil slides).

https://www.abcam.com/index.html?datasheet=4350 (or use the following: https://www.abcam.com/index.html?datasheet=4350). PC for ab25101and ab10563

https://www.abcam.com/index.html?datasheet=4348 (or use the following: https://www.abcam.com/index.html?datasheet=4348). PC for ab34843

https://www.abcam.com/index.html?datasheet=4372 (or use the following: https://www.abcam.com/index.html?datasheet=4372). PC for ab37158

Read More

Answer

Thank you for your enquiry. The immunogen for this antibody is conserved in isoforms A-D and the precursor of IL-32. Please let me know if you have any more questions.

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Answer

Thank you for your enquiry. Regarding ab37158 (IL32 antibody), this product has been tested on EL4 cell lysate. This cell lysate is available from Abcam (ab7183 - Mouse lymphoma cell line). I hope this information helps. Please don't hesitate to contact us again if you have further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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