Anti-IL-21抗体(ab5978)
Key features and details
- Rabbit polyclonal to IL-21
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-IL-21抗体
参阅全部 IL-21 一抗 -
描述
兔多克隆抗体to IL-21 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog, Pig
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免疫原
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阳性对照
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常规说明
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa. |
靶标
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功能
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
组织特异性
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
序列相似性
Belongs to the IL-15/IL-21 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 59067 Human
- Entrez Gene: 60505 Mouse
- Entrez Gene: 365769 Rat
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
- SwissProt: Q9ES17 Mouse
- SwissProt: A3QPB9 Rat
- Unigene: 567559 Human
see all -
别名
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
图片
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Western blot - Anti-IL-21 antibody (ab5978)All lanes : Anti-IL-21 antibody (ab5978) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.
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Western blot - Anti-IL-21 antibody (ab5978)Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1µ g /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. -
Western blot - Anti-IL-21 antibody (ab5978) -
Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)ab5978 at 2ug/ml staining of IL21 in K562 cells.
实验方案
数据表及文件
文献 (5)
ab5978 被引用在 5 文献中.
- Xue D et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
- Tan L et al. Chitosan nanoparticle-based delivery of fused NKG2D-IL-21 gene suppresses colon cancer growth in mice. Int J Nanomedicine 12:3095-3107 (2017). WB . PubMed: 28450784
- Liu T et al. Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis. Chin Med J (Engl) 130:2101-2106 (2017). PubMed: 28836555
- Qiu F et al. A genome-wide association study identifies six novel risk loci for primary biliary cholangitis. Nat Commun 8:14828 (2017). IHC-P ; Human . PubMed: 28425483
- Lee HK et al. Natural allelic variation of the IL-21 receptor modulates ischemic stroke infarct volume. J Clin Invest 126:2827-38 (2016). PubMed: 27400126
