Anti-IL-21抗体(ab5978)
Key features and details
- Rabbit polyclonal to IL-21
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-21抗体
参阅全部 IL-21 一抗 -
描述
兔多克隆抗体to IL-21 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Pig
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免疫原
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阳性对照
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常规说明
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa. |
靶标
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功能
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
组织特异性
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
序列相似性
Belongs to the IL-15/IL-21 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 59067 Human
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
- Unigene: 567559 Human
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别名
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
图片
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Western blot - Anti-IL-21 antibody (ab5978)
All lanes : Anti-IL-21 antibody (ab5978) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.
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Western blot - Anti-IL-21 antibody (ab5978)Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1µ g /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. -
Western blot - Anti-IL-21 antibody (ab5978) -
Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)ab5978 at 2ug/ml staining of IL21 in K562 cells.
实验方案
数据表及文件
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Datasheet download
文献 (9)
ab5978 被引用在 9 文献中.
- Wang N et al. A novel vaccinia virus enhances anti-tumor efficacy and promotes a long-term anti-tumor response in a murine model of colorectal cancer. Mol Ther Oncolytics 20:71-81 (2021). PubMed: 33575472
- Liu Z et al. Circulating follicular T helper cells and humoral reactivity in rheumatic heart disease. Life Sci 245:117390 (2020). PubMed: 32007574
- Liu Z et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
- Sadeghpour S et al. Effects of treatment with hydroxychloroquine on the modulation of Th17/Treg ratio and pregnancy outcomes in women with recurrent implantation failure: clinical trial. Immunopharmacol Immunotoxicol 42:632-642 (2020). PubMed: 33081532
- Xue D et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
- Tan L et al. Chitosan nanoparticle-based delivery of fused NKG2D-IL-21 gene suppresses colon cancer growth in mice. Int J Nanomedicine 12:3095-3107 (2017). WB . PubMed: 28450784
- Liu T et al. Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis. Chin Med J (Engl) 130:2101-2106 (2017). PubMed: 28836555
- Qiu F et al. A genome-wide association study identifies six novel risk loci for primary biliary cholangitis. Nat Commun 8:14828 (2017). IHC-P ; Human . PubMed: 28425483
- Lee HK et al. Natural allelic variation of the IL-21 receptor modulates ischemic stroke infarct volume. J Clin Invest 126:2827-38 (2016). PubMed: 27400126
