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Immunology Innate Immunity Cytokines Interleukins

Anti-IL-21抗体(ab5978)

  • Datasheet
Reviews (2) Submit a question References (9)

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Western blot - Anti-IL-21 antibody (ab5978)
  • Western blot - Anti-IL-21 antibody (ab5978)
  • Western blot - Anti-IL-21 antibody (ab5978)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)

Key features and details

  • Rabbit polyclonal to IL-21
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-IL-21 antibody [EPR4907] (ab178694)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-IL-21抗体
    参阅全部 IL-21 一抗
  • 描述

    兔多克隆抗体to IL-21
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human IL-21 aa 121-135.
    Sequence:

    TCPSCDSYEKKPPKE


    Database link: Q9HBE4
    (Peptide available as ab6025, ab6680)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • 购买相配的WB阳性对照Recombinant human IL-21 protein
  • 常规说明

    A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human IL-21 protein (ab60287)
    • THP-1 whole cell lysate (ab7913)
    • HL-60 whole cell lysate (ab7914)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5978于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 2 µg/ml.
WB (1)
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.
说明
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.

靶标

  • 功能

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • 组织特异性

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • 序列相似性

    Belongs to the IL-15/IL-21 family.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession Q9HBE4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 59067 Human
    • Omim: 605384 Human
    • SwissProt: Q9HBE4 Human
    • Unigene: 567559 Human
    • 别名

      • CVID11 antibody
      • IL 21 antibody
      • IL-21 antibody
      • Il21 antibody
      • IL21_HUMAN antibody
      • Interleukin 21 antibody
      • Interleukin-21 antibody
      • interleukin-21 isoform antibody
      • Interleukin21 antibody
      • OTTHUMP00000164088 antibody
      • Za11 antibody
      see all

    图片

    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)

      All lanes :  Anti-IL-21 antibody (ab5978) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.

      Loading: 15 μg of lysates per lane.

      Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.

    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)
      Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml.
    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)
      Anti-IL-21 antibody (ab5978) at 1 µg/ml + Recombinant human IL-21 protein (ab73250) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 8 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)
      ab5978 at 2ug/ml staining of IL21 in K562 cells.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (9)

    发表研究结果有使用 ab5978?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5978 被引用在 9 文献中.

    • Wang N  et al. A novel vaccinia virus enhances anti-tumor efficacy and promotes a long-term anti-tumor response in a murine model of colorectal cancer. Mol Ther Oncolytics 20:71-81 (2021). PubMed: 33575472
    • Liu Z  et al. Circulating follicular T helper cells and humoral reactivity in rheumatic heart disease. Life Sci 245:117390 (2020). PubMed: 32007574
    • Liu Z  et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
    • Sadeghpour S  et al. Effects of treatment with hydroxychloroquine on the modulation of Th17/Treg ratio and pregnancy outcomes in women with recurrent implantation failure: clinical trial. Immunopharmacol Immunotoxicol 42:632-642 (2020). PubMed: 33081532
    • Xue D  et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
    • Tan L  et al. Chitosan nanoparticle-based delivery of fused NKG2D-IL-21 gene suppresses colon cancer growth in mice. Int J Nanomedicine 12:3095-3107 (2017). WB . PubMed: 28450784
    • Liu T  et al. Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis. Chin Med J (Engl) 130:2101-2106 (2017). PubMed: 28836555
    • Qiu F  et al. A genome-wide association study identifies six novel risk loci for primary biliary cholangitis. Nat Commun 8:14828 (2017). IHC-P ; Human . PubMed: 28425483
    • Lee HK  et al. Natural allelic variation of the IL-21 receptor modulates ischemic stroke infarct volume. J Clin Invest 126:2827-38 (2016). PubMed: 27400126

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-21 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    MS. Hongyan Zhao

    Verified customer

    提交于 Feb 25 2014

    Western blot abreview for Anti-IL-21 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    60 µg
    Treatment
    transfection of plasmid expressing IL21
    Specification
    293T
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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