重组Anti-IL-2 Receptor alpha抗体[SP176] (ab231441)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP176] to IL-2 Receptor alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-2 Receptor alpha抗体[SP176]
参阅全部 IL-2 Receptor alpha 一抗 -
描述
兔单克隆抗体[SP176] to IL-2 Receptor alpha -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: MOLT-4, HDLM-2 and WEHI-231 whole cell lysate. IHC-P: Human endometrium and tonsil tissue. ICC/IF: HDLM-2 cells
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP176 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231441于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200. Predicted molecular weight: 31 kDa.
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IHC-P | (2) |
1/100.
Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF | (1) |
1/50.
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说明 |
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WB
1/200. Predicted molecular weight: 31 kDa. |
IHC-P
1/100. Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF
1/50. |
靶标
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功能
Receptor for interleukin-2. -
疾病相关
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3559 Human
- Entrez Gene: 16184 Mouse
- Entrez Gene: 25704 Rat
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P01590 Mouse
- SwissProt: P26897 Rat
- Unigene: 231367 Human
see all -
别名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
图片
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Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5µg/mL). Cells were fixed in 100% methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab231441 被引用在 5 文献中.
- Zhou Y et al. Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling. Front Immunol 13:859581 (2022). PubMed: 35795662
- Tian Q et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822
- Lee JH et al. Cysteine-specific 89Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas. Front Immunol 13:1017132 (2022). PubMed: 36591250
- Cao Q et al. miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ. Ann Transl Med 9:1410 (2021). PubMed: 34733962
- Tillman H et al. Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice. Vet Pathol 57:160-171 (2020). PubMed: 31736441