Anti-IL-2 Receptor alpha抗体[OX39] - BSA and Azide free (ab244557)
Key features and details
- Mouse monoclonal [OX39] to IL-2 Receptor alpha - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG1
概述
-
产品名称
Anti-IL-2 Receptor alpha抗体[OX39] - BSA and Azide free
参阅全部 IL-2 Receptor alpha 一抗 -
描述
小鼠单克隆抗体[OX39] to IL-2 Receptor alpha - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-Frmore details -
种属反应性
与反应: Rat, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cyt: Lewis rat splenocytes treated with 5 g/ml ConA for 3 days.IHC-Fr: Normal rat spleen tissue.
-
常规说明
ab244557 is the carrier-free version of ab6411.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
OX39 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab244557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 0.2 µg/ml.
|
|
IHC-Fr |
Use a concentration of 5 µg/ml.
|
说明 |
---|
Flow Cyt
Use a concentration of 0.2 µg/ml. |
IHC-Fr
Use a concentration of 5 µg/ml. |
靶标
-
功能
Receptor for interleukin-2. -
疾病相关
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3559 Human
- Entrez Gene: 25704 Rat
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P26897 Rat
- Unigene: 231367 Human
- Unigene: 9872 Rat
-
别名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
图片
-
This data was developed using the same antibody clone in a different buffer formulation (ab6411).
Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5 µg/ml ConA for 3 days (bottom) were stained with ab6411 (right) or mouse IgG1 kappa (ab170190) isotype (left).
Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6411) or mouse IgG1 kappa (ab170190) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.
-
This data was developed using the same antibody clone in a different buffer formulation (ab6411).
IHC image of IL2 receptor alpha staining in a section of frozen normal rat spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6411 at 5µg/ml and ab16669 (Rabbit monoclonal [SP7] to CD3) at 1/150. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor®488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab244557 尚未被引用在任何文献中。