Key features and details
- Mouse monoclonal [OX39] to IL-2 Receptor alpha
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
产品名称Anti-IL-2 Receptor alpha抗体[OX39]
参阅全部 IL-2 Receptor alpha 一抗
描述小鼠单克隆抗体[OX39] to IL-2 Receptor alpha
经测试应用适用于: IHC-Fr, Flow Cytmore details
Tissue, cells or virus corresponding to IL-2 Receptor alpha.
- Flow Cyt: Lewis rat splenocytes treated with 5 g/ml ConA for 3 days. IHC-Fr: Normal rat spleen tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab6411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
功能Receptor for interleukin-2.
疾病相关Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
序列相似性Contains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5 µg/ml ConA for 3 days (bottom) were stained with ab6411 (right) or mouse IgG1 kappa (ab170190) isotype (left).
Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6411) or mouse IgG1 kappa (ab170190) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.
IHC image of IL2 receptor alpha staining in a section of frozen normal rat spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6411 at 5µg/ml and ab16669 (Rabbit monoclonal [SP7] to CD3) at 1/150. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor®488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.