概述

  • 产品名称
    Anti-IL-15抗体[EP433Y]
    参阅全部 IL-15 一抗
  • 描述
    兔单克隆抗体[EP433Y] to IL-15
  • 宿主
    Rabbit
  • 特异性
    This antibody is specific for IL-15.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Human
  • 免疫原

    Recombinant full length protein within Human IL-15. The exact sequence is proprietary.

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab40668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 18 kDa).

靶标

  • 功能
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
  • 组织特异性
    Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
  • 序列相似性
    Belongs to the IL-15/IL-21 family.
  • 细胞定位
    Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 15 antibody
    • IL-15 antibody
    • IL15 antibody
    • IL15_HUMAN antibody
    • Interleukin 15 antibody
    • Interleukin-15 antibody
    • Interleukin15 antibody
    • MGC9721 antibody
    see all

图片

文献

ab40668 has not yet been referenced specifically in any publications.

客户评价及客户问答

Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have been looking into how the anti-IL15 antibodies ab40668 and ab134177 have been tested and whether they would be the most suitable antibodies to use in your experiments.

As discussed over the phone, the bands observed with ab40668 and ab134177 in Western blotting are not exactly where they would be expected to run as recombinant fusion proteins were used. With ab40668 as fusion protein of IL-15 (aa49-162, 12kDa) and Thioredoxin-1 (aa1-109, 12kDa), plus His tag (7kDa) was used, bringing the expected molecular weight to ˜ 31 kDa which is what is observed on the blot. The antibody ab134177 used a slightly truncated version of the IL15, making the expected band size ˜12 kDa (as observed).

Unfortunately, both of these antibodies have been tried with samples containing endogenously expressed IL15 and we have been unable to detect a signal. It may be that the conditions necessary for using these antibodies with endogenous samples requires some further optimisation. I would suggest, as you are intending to use the antibodies with endogenous samples to have a look at the ab7213 or ab109082 instead. Both of these would be covered by the Abpromise guarantee for the application and species you intend to use the antibody for.

More information on the Abpromise can be found from the following link:

www.abcam.com/abpromise

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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