概述

  • 产品名称
    Anti-IL-12抗体(Biotin)
    参阅全部 IL-12 一抗
  • 描述
    山羊多克隆抗体to IL-12 (Biotin)
  • 宿主
    Goat
  • 偶联物
    Biotin
  • 经测试应用
    适用于: ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein corresponding to Mouse IL-12.

  • 阳性对照
    • Murine IL-12

性能

应用

Our Abpromise guarantee covers the use of ab83449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Sandwich ELISA
WB
  • 应用说明
    ELISA: Use at a concentration of 0.25 - 1.0 µg/ml.
    sELISA: Use at a concentration of 0.25 - 1.0 µg/ml.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight of mouse IL-12: 24 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    • 序列相似性
      Belongs to the IL-6 superfamily.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CLMF antibody
      • CLMF p35 antibody
      • CLMF p40 antibody
      • CLMF1 antibody
      • CLMF2 antibody
      • Cytotoxic lymphocyte maturation factor 1, p35 antibody
      • Cytotoxic lymphocyte maturation factor 2 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
      • IL 2 subunit p40 antibody
      • IL-12 subunit p35 antibody
      • IL-12A antibody
      • IL-12B antibody
      • IL12 antibody
      • IL12, p35 subunit antibody
      • IL12, subunit p40 antibody
      • IL12A antibody
      • IL12A_HUMAN antibody
      • IL12B antibody
      • IL35 subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin 12 beta chain antibody
      • Interleukin 12 subunit beta antibody
      • Interleukin 12, p40 antibody
      • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
      • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody
      • Interleukin 12B antibody
      • Interleukin-12 subunit alpha antibody
      • Natural killer cell stimulatory factor 1, 35 kD subunit antibody
      • natural killer cell stimulatory factor 2 antibody
      • Natural killer cell stimulatory factor, 40 kD subunit antibody
      • NF cell stimulatory factor chain 1 antibody
      • NFSK antibody
      • NK cell stimulatory factor chain 1 antibody
      • NK cell stimulatory factor chain 2 antibody
      • NKSF antibody
      • NKSF1 antibody
      • NKSF2 antibody
      • P35 antibody
      see all

    实验方案

    文献

    ab83449 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us.

    During the chemical conjugation process, biotin is non-specifically attached to free groups on the antibody and multiple biotins will conjugate to a single antibody. For this reason, there is no single site at which biotin is attached to this antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

    Read More

    Answer

    ab83449: DISCOUNT CODE: 00000000

    ab91217: DISCOUNT CODE:00000



    Ablaufdatum: 07. Oktober 2012

    Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab83449 und ab91217insELISA zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Ein Discountcode deckt den Wert vonje einem Antikörperab und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in sELISA eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

    Read More

    Answer

    Thank you for your inquiry.

    I can confirm that you will receive two discount codes when testing two antibodies in sandwich ELISA that have not been previously tested for it.

    These discount codes will be valid after submitting an Abreview about the results of the sandwich ELISA whether they are positive or negative. We value any information and believe that also our other users appreciate honest information.

    Unfortunately, we do not offer substrate solutions for ELISA. To my knowledge 3,3’,5,5’-tetramethylbenzidine (TMB) is generally used for HRP ELISAs.

    But I am happy to confirm that we have Streptavidin Peroxidase in our catalog:

    ab64269:

    https://www.abcam.com/index.html?datasheet=64269 (or use the following: https://www.abcam.com/index.html?datasheet=64269).

    We also offer easy to use ELISA kits for IL12:

    ab119531

    https://www.abcam.com/index.html?datasheet=119531 (or use the following: https://www.abcam.com/index.html?datasheet=119531).

    and ab100699

    https://www.abcam.com/index.html?datasheet=100699 (or use the following: https://www.abcam.com/index.html?datasheet=100699).

    Please let me know if you would like to go forward and test ab83449 and ab91217 in sandwich ELISA and I will be happy to issue the two codes for you.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册