Application
Western blot
Sample
Human Cell lysate - whole cell (human peripheral blood mononuclear cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100 µg
Treatment
5hr PMA (50 ng/mL) + Ionomycin (1 ug/mL) + monensin 2uM
Specification
human peripheral blood mononuclear cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/2500
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO rabbit anti goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Plus
Exposure
12 second(s)
Bands
Specific: 70, 40, 35 kDa Non-specific: 150 kDa
Positive control
this sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 06 2011