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Immunology Innate Immunity Cytokines Interleukins

Anti-IL-1 beta抗体(ab2105)

  • Datasheet
  • SDS
Reviews (23)Q&A (17)References (113)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)

Key features and details

  • Rabbit polyclonal to IL-1 beta
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-IL-1 beta antibody [EPR21086] (ab216995)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-IL-1 beta抗体
    参阅全部 IL-1 beta 一抗
  • 描述

    兔多克隆抗体to IL-1 beta
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 17kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).

  • 阳性对照

    • IHC-P: human medullary lymph node
  • 常规说明

    caution, F(c) domain of rabbit IgG may interact with cells non-specifically.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    IgG from whole rabbit serum purified by DEAE fractionation.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Obesity

相关产品

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    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IL-1 beta protein (Active) (ab259387)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2105于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (9)
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 组织特异性

    Expressed in activated monocytes/macrophages (at protein level).
  • 序列相似性

    Belongs to the IL-1 family.
  • 翻译后修饰

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 细胞定位

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3553 Human
    • Omim: 147720 Human
    • SwissProt: P01584 Human
    • Unigene: 126256 Human
    • 别名

      • Catabolin antibody
      • H1 antibody
      • IFN beta inducing factor antibody
      • IL 1 antibody
      • IL 1 beta antibody
      • IL-1 beta antibody
      • IL1 antibody
      • IL1 BETA antibody
      • IL1B antibody
      • IL1B_HUMAN antibody
      • IL1F2 antibody
      • Interleukin 1 beta antibody
      • Interleukin 1 beta precursor antibody
      • interleukin 1, beta antibody
      • Interleukin-1 beta antibody
      • OAF antibody
      • Osteoclast activating factor antibody
      • OTTHUMP00000162031 antibody
      • Preinterleukin 1 beta antibody
      • Preinterleukin beta antibody
      • Pro interleukin 1 beta antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemical analysis of Formalin-fixed paraffin-embedded human medullary lymph node sections labeling IL-1 beta with ab2105. Peroxidase goat anti-rabbit at 1/10,000 was used as the secondary antibody. 
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)This image is courtesy of an Abreview submitted by Miss Silke Vorwald
      ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (113)

    发表研究结果有使用 ab2105?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2105 被引用在 113 文献中.

    • Qi S  et al. P38 MAPK signaling pathway mediates COM crystal-induced crystal adhesion change in rat renal tubular epithelial cells. Urolithiasis 48:9-18 (2020). PubMed: 31183507
    • Peng B  et al. Role of interleukin-1ß in nerve growth factor expression, neurogenesis and deep dyspareunia in endometriosis. Hum Reprod 35:901-912 (2020). PubMed: 32240297
    • Wang Y  et al. Linkage of lncRNA CRNDE sponging miR-181a-5p with aggravated inflammation underlying sepsis. Innate Immun 26:152-161 (2020). PubMed: 31604377
    • Chao S  et al. Polygala fallax Hemsl combined with compound Sanqi granules relieves glomerulonephritis by regulating proliferation and apoptosis of glomerular mesangial cells. J Int Med Res 48:300060519894124 (2020). PubMed: 32000547
    • Zhang J  et al. MicroRNA-483 amelioration of experimental pulmonary hypertension. EMBO Mol Med 12:e11303 (2020). PubMed: 32324970
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, stomach, esophagus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    Yes - Tween 20 0.1%
    Specification
    brain, stomach, esophagus
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 26 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (chondrocytes)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    No
    Specification
    chondrocytes
    Blocking step
    Bloxall and Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More

    Mrs. Merissa Olmer

    Verified customer

    提交于 Jun 08 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (cartilage)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    No
    Specification
    cartilage
    Blocking step
    h2o2, then serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution, ph6.0
    Permeabilization
    No
    Specification
    Colon
    Fixative
    Formaldehyde
    Read More

    Pan Pan

    Verified customer

    提交于 Aug 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    Macaque retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 10 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
    Permeabilization
    Yes - 0,1% Saponin/PBS
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 11 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Shoulder tissue)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Shoulder tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Ms. Mindy Cote

    Verified customer

    提交于 Dec 15 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Shoulder)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Shoulder
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Ms. Mindy Cote

    Verified customer

    提交于 Aug 23 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (liver)
    Antigen retrieval step
    Heat mediated
    Specification
    liver
    Blocking step
    No blocking step used for 10 minute(s) · Concentration: 1%
    Fixative
    Paraformaldehyde
    Read More

    Miss. Silke Vorwald

    Verified customer

    提交于 Mar 09 2007

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