重组Anti-IL-1 alpha抗体[EPR19904-247] (ab206410)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19904-247] to IL-1 alpha
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IL-1 alpha抗体[EPR19904-247]
参阅全部 IL-1 alpha 一抗 -
描述
兔单克隆抗体[EPR19904-247] to IL-1 alpha -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human IL-1 alpha recombinant protein (aa113-271); THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, cell lysate. Flow Cyt (intra): THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19904-247 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206410于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/60.
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WB |
1/1000. Detects a band of approximately 34, 20, 15 kDa (predicted molecular weight: 31 kDa).
|
说明 |
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Flow Cyt (Intra)
1/60. |
WB
1/1000. Detects a band of approximately 34, 20, 15 kDa (predicted molecular weight: 31 kDa). |
靶标
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功能
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
序列相似性
Belongs to the IL-1 family. -
结构域
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
细胞定位
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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数据库链接
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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别名
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
图片
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green)labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 15,20,34 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID: 9785472; PMID: 1868253; PMID:19104081).
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Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution + Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab206410 被引用在 4 文献中.
- Chung AW et al. Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6. NPJ Breast Cancer 8:30 (2022). PubMed: 35260569
- Kalbassi S et al. A Comparison of the Characteristics of Cytokine Storm between Lichen Planus and Oral Squamous Cell Carcinoma. Asian Pac J Cancer Prev 23:3843-3849 (2022). PubMed: 36444597
- Wang X et al. A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis. Cell Death Dis 12:402 (2021). PubMed: 33854044
- Wang X et al. An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling. Front Pharmacol 12:673818 (2021). PubMed: 34093202