重组Anti-IKZF3抗体[EPR9342(B)] (ab139408)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9342(B)] to IKZF3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-IKZF3抗体[EPR9342(B)]
参阅全部 IKZF3 一抗 -
描述
兔单克隆抗体[EPR9342(B)] to IKZF3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human IKZF3 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available asab184024) -
阳性对照
- WB: Recombinant human IKZF3 protein (ab132618); Human thymus, Jurkat, Raji, and Ramos cell lysates. IHC-P: Human and rat spleen tissues and mouse cardiac muscle tissue. ICC/IF: Jurkat cells. Flow Cyt (intra): Ramos cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9342(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab139408于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/20000. Predicted molecular weight: 58 kDa.
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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ICC/IF |
1/100.
For unpurified use at 1/250 - 1/500. |
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/20000. Predicted molecular weight: 58 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ICC/IF
1/100. For unpurified use at 1/250 - 1/500. |
靶标
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功能
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. -
组织特异性
Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. -
序列相似性
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
翻译后修饰
Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 22806 Human
- Entrez Gene: 22780 Mouse
- Entrez Gene: 303511 Rat
- Omim: 606221 Human
- SwissProt: Q9UKT9 Human
- SwissProt: O08900 Mouse
- Unigene: 444388 Human
- Unigene: 133367 Mouse
see all -
别名
- AIO antibody
- Aiolos antibody
- IKAROS family zinc finger 3 (Aiolos) antibody
see all
图片
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All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/20000 dilution (purified)
Lane 1 : Raji (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labeling IKZF3 with purified ab139408 at 1/100.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling IKZF3 (green) with purified ab139408 at 1/100.
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: Secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
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Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma cell line cells labeling IKZF3 with purified ab139408 at 1/90 (red).
Cells were fixed with 2% paraformaldehyde. An FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
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All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/1000 dilution (unpurified)
Lane 1 : Human thymus cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling IKZF3 with purified ab139408 at 1/100.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labeling IKZF3 with purified ab139408 at 1/100.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling IKZF3 with unpurified ab139408 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab139408 被引用在 9 文献中.
- Vecellio M et al. Functional Genomic Analysis of a RUNX3 Polymorphism Associated With Ankylosing Spondylitis. Arthritis Rheumatol 73:980-990 (2021). PubMed: 33369221
- Verhoeven RJA et al. Epstein-Barr Virus BART Long Non-coding RNAs Function as Epigenetic Modulators in Nasopharyngeal Carcinoma. Front Oncol 9:1120 (2019). PubMed: 31696060
- Lu X et al. MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition. Cell Rep 28:472-485.e5 (2019). PubMed: 31291582
- Li S et al. IMiD compounds affect CD34+ cell fate and maturation via CRBN-induced IKZF1 degradation. Blood Adv 2:492-504 (2018). WB . PubMed: 29496670
- Liu J et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). PubMed: 30026574
- Mori T et al. Structural basis of thalidomide enantiomer binding to cereblon. Sci Rep 8:1294 (2018). PubMed: 29358579
- Nakayama Y et al. Aiolos Overexpression in Systemic Lupus Erythematosus B Cell Subtypes and BAFF-Induced Memory B Cell Differentiation Are Reduced by CC-220 Modulation of Cereblon Activity. J Immunol 199:2388-2407 (2017). PubMed: 28848067
- Jian Y et al. Arsenic trioxide potentiates sensitivity of multiple myeloma cells to lenalidomide by upregulating cereblon expression levels. Oncol Lett 14:3243-3248 (2017). PubMed: 28927072
- Evans HG et al. TNF-a blockade induces IL-10 expression in human CD4+ T cells. Nat Commun 5:3199 (2014). PubMed: 24492460