Anti-IKKi/IKKe抗体(ab7891)
Key features and details
- Rabbit polyclonal to IKKi/IKKe
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-IKKi/IKKe抗体
参阅全部 IKKi/IKKe 一抗 -
描述
兔多克隆抗体to IKKi/IKKe -
宿主
Rabbit -
特异性
Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma. -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified IgG was prepared by affinity chromatography. -
Primary antibody说明
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
靶标
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功能
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. -
组织特异性
Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1. -
细胞定位
Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent. - Information by UniProt
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数据库链接
- Entrez Gene: 9641 Human
- Entrez Gene: 56489 Mouse
- Omim: 605048 Human
- SwissProt: Q14164 Human
- SwissProt: Q9R0T8 Mouse
- Unigene: 321045 Human
- Unigene: 386783 Mouse
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别名
- I kappa B kinase epsilon antibody
- I-kappa-B kinase epsilon antibody
- IkBKE antibody
see all
图片
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Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : Untreated Raw264.7 cell lysates
Lane 2 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
Lane 3 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa -
Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SK-N-SH cell lysate
Lane 5 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa -
Western blot - Anti-IKKi/IKKe antibody (ab7891)Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC.
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Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (14)
ab7891 被引用在 14 文献中.
- Tseng AC et al. Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication. Viruses 13:N/A (2021). PubMed: 33572517
- Liu G et al. IKKe phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics 10:2358-2373 (2020). PubMed: 32104508
- Qiao J et al. Macrophages confer resistance to BET inhibition in triple-negative breast cancer by upregulating IKBKE. Biochem Pharmacol 180:114126 (2020). PubMed: 32603665
- Yuan Y et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). PubMed: 30794866
- Wang X et al. Expression and prognostic role of IKBKE and TBK1 in stage I non-small cell lung cancer. Cancer Manag Res 11:6593-6602 (2019). PubMed: 31406474
