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Cell Biology Apoptosis Intracellular NFkB IKK

Anti-IKKi/IKKe抗体(ab7891)

  • Datasheet
Reviews (1)Q&A (1)References (14)

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Western blot - Anti-IKKi/IKKe antibody (ab7891)
  • Western blot - Anti-IKKi/IKKe antibody (ab7891)
  • Western blot - Anti-IKKi/IKKe antibody (ab7891)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)

Key features and details

  • Rabbit polyclonal to IKKi/IKKe
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant human IKKi/IKKe protein (ab201367)

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概述

  • 产品名称

    Anti-IKKi/IKKe抗体
    参阅全部 IKKi/IKKe 一抗
  • 描述

    兔多克隆抗体to IKKi/IKKe
  • 宿主

    Rabbit
  • 特异性

    Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma.
  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Purified IgG was prepared by affinity chromatography.
  • Primary antibody说明

    Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • IKK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • IKK

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human IKKi/IKKe protein (ab201367)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7891于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
说明
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.

靶标

  • 功能

    Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
  • 组织特异性

    Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
  • 序列相似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰

    Autophosphorylated.
    Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
  • 细胞定位

    Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
  • Target information above from: UniProt accession Q14164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9641 Human
    • Entrez Gene: 56489 Mouse
    • Omim: 605048 Human
    • SwissProt: Q14164 Human
    • SwissProt: Q9R0T8 Mouse
    • Unigene: 321045 Human
    • Unigene: 386783 Mouse
    • 别名

      • I kappa B kinase epsilon antibody
      • I-kappa-B kinase epsilon antibody
      • IkBKE antibody
      • IKK related kinase epsilon antibody
      • IKK-E antibody
      • IKK-epsilon antibody
      • IKK-i antibody
      • IKKE antibody
      • IKKE_HUMAN antibody
      • IKKepsilon antibody
      • IKKI antibody
      • Inducible I kappa B kinase antibody
      • Inducible I kappa-B kinase antibody
      • Inducible IkappaB kinase antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
      • KIAA0151 antibody
      • MGC125294 antibody
      • MGC125295 antibody
      • MGC125297 antibody
      see all

    图片

    • Western blot - Anti-IKKi/IKKe antibody (ab7891)
      Western blot - Anti-IKKi/IKKe antibody (ab7891)
      All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml

      Lane 1 : Untreated Raw264.7 cell lysates
      Lane 2 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
      Lane 3 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 80 kDa

    • Western blot - Anti-IKKi/IKKe antibody (ab7891)
      Western blot - Anti-IKKi/IKKe antibody (ab7891)
      All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml

      Lane 1 : A431 cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : SK-N-SH cell lysate
      Lane 5 : 3T3/NIH cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 80 kDa

    • Western blot - Anti-IKKi/IKKe antibody (ab7891)
      Western blot - Anti-IKKi/IKKe antibody (ab7891)
      Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate

      Predicted band size: 80 kDa
      Observed band size: 80 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)

      ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC.

    • Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
      Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)

      ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
      Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)

      Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20µg/ml.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab7891?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7891 被引用在 14 文献中.

    • Tseng AC  et al. Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication. Viruses 13:N/A (2021). PubMed: 33572517
    • Liu G  et al. IKKe phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics 10:2358-2373 (2020). PubMed: 32104508
    • Qiao J  et al. Macrophages confer resistance to BET inhibition in triple-negative breast cancer by upregulating IKBKE. Biochem Pharmacol 180:114126 (2020). PubMed: 32603665
    • Yuan Y  et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). PubMed: 30794866
    • Wang X  et al. Expression and prognostic role of IKBKE and TBK1 in stage I non-small cell lung cancer. Cancer Manag Res 11:6593-6602 (2019). PubMed: 31406474
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-IKKi/IKKe antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Loading amount
    15 µg
    Specification
    MCF7
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 12 2009

    Question

    I was wondering if this antibody will cross-react with mouse ikk epsilon

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2001

    Answer

    This antibody has not yet been tested with mouse ikk epsilon.

    Read More

    Abcam Scientific Support

    回复于 Nov 01 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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