Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1 µg/ml
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Pierce31210
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
NEMO knock-out (KO) MEFs
Exposure
5 second(s)
Bands
Specific: 45 kDa Non-specific: 55, 70, 77 kDa
Positive control
NEMO wild-type (WT) MEFs
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 24 2014