Anti-IKK beta抗体(ab97406)
Key features and details
- Rabbit polyclonal to IKK beta
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IKK beta抗体
参阅全部 IKK beta 一抗 -
描述
兔多克隆抗体to IKK beta -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Monkey -
免疫原
Recombinant fragment corresponding to Human IKK beta aa 450-750.
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阳性对照
- WB: Whole cell extract of DDDDK-human IKBKB-transfected 293T cells. IHC-: Human colon carcinoma tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97406于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. -
组织特异性
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
细胞定位
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 281854 Cow
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: Q95KV0 Cow
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
see all -
别名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
图片
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All lanes : Anti-IKK beta antibody (ab97406) at 1/5000 dilution
Lane 1 : 293T whole cell extract
Lane 2 : Whole cell extract of DDDDK-human IKBKB-transfected 293T cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Anti-IKK beta antibody (ab97406) at 1/1000 dilution + PC-12 whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 87 kDa
Observed band size: 87 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling IKK beta with ab97406 at 1/500 dilution. Antigen retrieval was performed with an EDTA based buffer (pH 9.0) for 15 mins.
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ab97406 at 1/200 dilution staining IKK beta in A431 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab97406 被引用在 5 文献中.
- Zhang J et al. Knockdown of triggering receptor expressed on myeloid cells 1 (TREM1) inhibits endoplasmic reticulum stress and reduces extracellular matrix degradation and the apoptosis of human nucleus pulposus cells. Exp Ther Med 24:607 (2022). PubMed: 36160910
- Wang Z et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370
- Gao Y et al. Semaphorin 3A contributes to sepsis-induced immunosuppression by impairing CD4+ T cell anergy. Mol Med Rep 23:N/A (2021). PubMed: 33649856
- Liu Y et al. Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression. Biomed Eng Online 20:67 (2021). PubMed: 34217312
- Dong Y et al. Maternally-Expressed Gene 3 (MEG3)/miR-143-3p Regulates Injury to Periodontal Ligament Cells by Mediating the AKT/Inhibitory ?B Kinase (IKK) Pathway. Med Sci Monit 26:e922486 (2020). PubMed: 32520926