Application
Western blot
Sample
Guinea pig Tissue lysate - whole (myometrium pregnant and non-pregnant)
Loading amount
30 µg
Specification
myometrium pregnant and non-pregnant
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL prime
Exposure
30 second(s)
Bands
Specific: none kDa Non-specific: 85, 53, 30 kDa
Positive control
30 ug of each: human myometrium non-preganat and pregnant, WISH cells LPS treated, HeLa cells these cells show a strong band of approx. 88 kDA, plus unspecific bands 85, 53,40,30kDa, see the image
Additional data
Additional Notes
samples: 1) myometrium of non-pregnant guinea pig 30ug
2) myometrium of pregnant guinea pig 30ug
3) human non-pregnant myometrium 30ug
4) human pregnant myometrium 30ug
5) wish cells treated LPS 30ug
6) HeLa cells 30 ug
2) myometrium of pregnant guinea pig 30ug
3) human non-pregnant myometrium 30ug
4) human pregnant myometrium 30ug
5) wish cells treated LPS 30ug
6) HeLa cells 30 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 14 2011