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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
使用敲除细胞株进行验证重组RabMAb

重组Anti-IKB alpha抗体[E130] (ab32518)

  • Datasheet
  • SDS
Reviews (30)Q&A (6)References (207)

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Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
  • Flow Cytometry - Anti-IKB alpha antibody [E130] (ab32518)
  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
  • Anti-IKB alpha antibody [E130] (ab32518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E130] to IKB alpha
  • Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-IKB alpha抗体[E130]
    参阅全部 IKB alpha 一抗
  • 描述

    兔单克隆抗体[E130] to IKB alpha
  • 宿主

    Rabbit
  • 特异性

    This antibody detects both the phosphorylated and non-phosphorylated form of the serine 32 region of IKB alpha.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human IKB alpha. The exact sequence is proprietary.
    Database link: P25963

  • 阳性对照

    • WB: Wild type HAP1 whole cell lysate. HeLa whole cell lysate. PC-12, SH-SY5Y, HepG2, K562, NIH/3T3 and RAW 264.7 cell lysate. Human fetal liver lysate. IHC-P: Human prostate carcinoma and stomach tissue. Rat and mouse kidney tissue. ICC/IF: HeLa and RAW 264.7 cells. IP: IKB alpha IP in HeLa cell lysate. PC-12 whole cell lysate. Flow Cyt: HeLa cells.
  • 常规说明

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    E130
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-IKB alpha antibody [E130] (ab202644)
    • HRP Anti-IKB alpha antibody [E130] (ab202646)
    • Alexa Fluor® 647 Anti-IKB alpha antibody [E130] (ab202647)
    • Alexa Fluor® 594 Anti-IKB alpha antibody [E130] (ab206346)
    • Alexa Fluor® 555 Anti-IKB alpha antibody [E130] (ab206578)
    • Anti-IKB alpha antibody [E130] - BSA and Azide free (ab215972)
    • APC Anti-IKB alpha antibody [E130] (ab224948)
    • PE Anti-IKB alpha antibody [E130] (ab224949)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IKB alpha protein (ab113133)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • FK506 (Tacrolimus), Immunosuppressant (ab120223)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Rat
Human
IP
Human
WB
Rat
Human
All applications
Hamster
Cow
Pig
应用 Ab评论 说明
ICC/IF
1/50.
WB (26)
1/1000 - 1/100000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP (2)
1/20.
Flow Cyt
1/20.
说明
ICC/IF
1/50.
WB
1/1000 - 1/100000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/20.
Flow Cyt
1/20.

靶标

  • 功能

    Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • 疾病相关

    Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • 序列相似性

    Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • 翻译后修饰

    Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • 细胞定位

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Target information above from: UniProt accession P25963 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 282291 Cow
    • Entrez Gene: 4792 Human
    • Entrez Gene: 18035 Mouse
    • Entrez Gene: 406188 Pig
    • Entrez Gene: 25493 Rat
    • Omim: 164008 Human
    • SwissProt: P25963 Human
    • SwissProt: Q9Z1E3 Mouse
    • SwissProt: Q08353 Pig
    • SwissProt: Q63746 Rat
    • Unigene: 81328 Human
    • Unigene: 170515 Mouse
    • Unigene: 12550 Rat
    see all
  • 别名

    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

图片

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: IKB alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab32518 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32518 was shown to specifically react with IKB alpha in wild-type HAP1 cells. No band was observed when IKB alpha knockout samples were tested. Wild-type and IKB alpha knockout samples were subjected to SDS-PAGE. ab32518 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)

    Immunohistochemical staining of paraffin embedded human stomach with purified ab32518 at a working dilution of 1 in 100.

    The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
    Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)

    Unpurified ab32518 staining IKB alpha in RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with FK506 (ab120223), by ICC/IF. Decrease in IkBα/&beta expression correlates with increased concentration of FK506, as described in literature.
    The cells were incubated at 37°C for 3h in media containing different concentrations of ab120223 (FK506) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature.

    Staining of the treated cells with ab32518 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
    Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)

    Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with purified ab32518 at a working dilution of 1 in 50, counterstained with DAPI.

    The secondary antibody was ab150077, Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

    The negative control is shown in bottom right hand panel - for the negative control, purified ab32518 was used at a dilution of 1/50 followed by ab150120, Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.

  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
    Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)

    ab32518 (purified) at 1/20 immunoprecipitating IKB alpha in HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (Lane 1). For western blotting a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    All lanes : Anti-IKB alpha antibody [E130] (ab32518) at 1/10000 dilution (purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using unpurified ab32518 at 1/50 dilution.

  • Flow Cytometry - Anti-IKB alpha antibody [E130] (ab32518)
    Flow Cytometry - Anti-IKB alpha antibody [E130] (ab32518)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKB alpha with purified ab32518 at 1/20 dilution (10 µg/mL) (red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    All lanes : Anti-IKB alpha antibody [E130] (ab32518) at 1/10000 dilution (purified)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : K562 Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab32518 at a working dilution of 1 in 100.

    The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)
    Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody [E130] (ab32518)

    ICC/IF image of unpurified ab32518 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h.

    DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Anti-IKB alpha antibody [E130] (ab32518) at 1/1000 dilution (purified) + Human fetal liver at 10 µg

    Secondary
    HRP anti-rabbit IgG, specific to the non reduced form of IgG at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Anti-IKB alpha antibody [E130] (ab32518) at 1/1000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Western blot - Anti-IKB alpha antibody [E130] (ab32518)
    Anti-IKB alpha antibody [E130] (ab32518) at 1/10000 dilution (unpurified) + HeLa cell lysate

    Predicted band size: 36 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
    Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)This image is courtesy of an anonymous Abreview

    Unpurified ab32518 used to immunoprecipitate IKB alpha from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate. The antibody was further used to Western blot the protein.

    Lane 1: IKB alpha IP

    Lane 2: Control immunoprecipitate

    Lane 3: Input (20%)

    See Abreview

  • Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)
    Immunoprecipitation - Anti-IKB alpha antibody [E130] (ab32518)This image is courtesy of an anonymous Abreview

    Unpurified ab32518 used to immunoprecipitate IKB alpha from PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate. The antibody was further used to Western blot the protein.

    Lane 1: IKB alpha IP

    Lane 2: Control immunoprecipitate

    Lane 3: Input (20%)

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody [E130] (ab32518)This image is courtesy of an anonymous Abreview

    Unpurified ab32518 at 1/100 staining mouse kidney tissue sections by IHC-P.

    The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-IKB alpha antibody [E130] (ab32518)
    Anti-IKB alpha antibody [E130] (ab32518)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (207)

    发表研究结果有使用 ab32518?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab32518 被引用在 207 文献中.

    • Sun X  et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
    • Zhang L  et al. TRIM22 inhibits endometrial cancer progression through the NOD2/NF-?B signaling pathway and confers a favorable prognosis. Int J Oncol 56:1225-1239 (2020). PubMed: 32319602
    • Liu H  et al. Copper induces hepatic inflammatory responses by activation of MAPKs and NF-?B signalling pathways in the mouse. Ecotoxicol Environ Saf 201:110806 (2020). PubMed: 32512418
    • Li L  et al. MicroRNA-584 Impairs Cellular Proliferation and Sensitizes Osteosarcoma Cells to Cisplatin and Taxanes by Targeting CCN2. Cancer Manag Res 12:2577-2587 (2020). PubMed: 32346311
    • Nam JE  et al. Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu. Life Sci 254:117742 (2020). PubMed: 32360619
    View all Publications for this product

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    Western blot abreview for Anti-IKB alpha antibody [E130]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 27 2019

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Endothelial)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Endothelial
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 28 2017

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 18 2016

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    28 min IRI
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Wesley Konsavage

    Verified customer

    提交于 Sep 15 2015

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human head and neck cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    10 nM PMA
    Specification
    human head and neck cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 31 2015

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle (AT))
    Specification
    Skeletal muscle (AT)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 27 2014

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2013

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2013

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2013

    Western blot abreview for Anti-IKB alpha antibody [E130]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (smooth muscle cells)
    Loading amount
    25 µg
    Specification
    smooth muscle cells
    Treatment
    infection with interested gene for 48 hours
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 16 2013

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