Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% Milk in TSB-T
Secondary antibody
Name
Dilution
1/2500
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 37 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Fabio Marongiu
Verified customer
提交于 Oct 11 2012