Application
Western blot
Sample
Mouse Cell lysate - other (NIH 3T3 Mouse Fibroblasts)
Loading amount
20 µg
Specification
NIH 3T3 Mouse Fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
Additional data
Additional Notes
Lane 1 - Bio-Rad WesternC Protein Standards
Lane 2 - 20ug NIH 3T3 lysate
Lane 2 - 20ug NIH 3T3 lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 24 2010