Anti-IGFBP2抗体(ab227881)
Key features and details
- Rabbit polyclonal to IGFBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IGFBP2抗体
参阅全部 IGFBP2 一抗 -
描述
兔多克隆抗体to IGFBP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Pig -
免疫原
Recombinant fragment within Human IGFBP2 (internal sequence). The exact sequence is proprietary.
Database link: P18065 -
阳性对照
- ICC/IF: Huh-7 cells. IHC-P: Human breast carcinoma tissue. WB: SK-N-AS whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
|
说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
结构域
The C-terminus is required for IGF-binding and growth inhibition. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3485 Human
- Entrez Gene: 397064 Pig
- Omim: 146731 Human
- SwissProt: P18065 Human
- SwissProt: P24853 Pig
- Unigene: 438102 Human
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别名
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
图片
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Anti-IGFBP2 antibody (ab227881) at 1/1000 dilution + SK-N-AS whole cell lysate at 30 µg
Predicted band size: 35 kDa10 % SDS-PAGE gel.
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Huh-7 cells stained for IGFBP2 (green) using ab227881 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Paraffin-embedded human breast carcinoma tissue stained for IGFBP2 using ab227881 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab227881 被引用在 1 文献中.
- Brockway HM et al. Unique transcriptomic landscapes identified in idiopathic spontaneous and infection related preterm births compared to normal term births. PLoS One 14:e0225062 (2019). PubMed: 31703110