为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体(ab5681)

  • Datasheet
Reviews (1)Q&A (2)References (9)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)

    Key features and details

    • Rabbit polyclonal to IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)
    • Suitable for: WB
    • Reacts with: Human, Recombinant fragment
    • Isotype: IgG

    你可能会感兴趣

    蛋白
    Product image
    Recombinant human IGF1 Receptor protein (ab155622)
    二抗
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    敲除
    Product image
    Human IGF1R (IGF1 Receptor) knockout HeLa cell line (ab264801)

    查看更多相关产品

    概述

    • 产品名称

      Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)抗体
      参阅全部 IGF1 Receptor 一抗
    • 描述

      兔多克隆抗体to IGF1 Receptor (phospho Y1158 + Y1162 + Y1163)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human, Recombinant fragment
      预测可用于: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to IGF1 Receptor (phospho Y1158 + Y1162 + W1163).

    • 阳性对照

      • WB: CHO-T (Chinese hamster ovary cell line) whole cell extract transfected with a vector encoding the human insulin receptor and stimulated with insulin.
    • 常规说明

      The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761. There are a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor (IR). The final product is generated by affinity chromatography using an IR-derived peptide phosphorylated at tyrosines 1158, 1162 and 1163 (1131, 1135 and 1136 for IGF1R).
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Neuroscience
      • Neurology process
      • Metabolism
      • Cancer
      • Growth factors
      • Insulin and insulin-like
      • Developmental Biology
      • Post embryonic development
      • Aging
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human IGF1 Receptor protein (ab155622)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab5681于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    1/1000. Detects a band of approximately 100 kDa.
    说明
    WB
    1/1000. Detects a band of approximately 100 kDa.

    靶标

    • 功能

      Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.
      When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
    • 组织特异性

      Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.
    • 疾病相关

      Insulin-like growth factor 1 resistance
    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰

      Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner; Tyr-1165 is predominantly phosphorylated first, followed by phosphorylation of Tyr-1161 and Tyr-1166. While every single phosphorylation increases kinase activity, all three tyrosine residues in the kinase activation loop (Tyr-1165, Tyr-1161 and Tyr-1166) have to be phosphorylated for optimal activity. Can be autophosphorylated at additional tyrosine residues (in vitro). Autophosphorylated is followed by phosphorylation of juxtamembrane tyrosines and C-terminal serines. Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. Phosphorylation of Ser-1278 by GSK-3beta restrains kinase activity and promotes cell surface expression, it requires a priming phosphorylation at Ser-1282. Dephosphorylated by PTPN1.
      Polyubiquitinated at Lys-1168 and Lys-1171 through both 'Lys-48' and 'Lys-29' linkages, promoting receptor endocytosis and subsequent degradation by the proteasome. Ubiquitination is facilitated by pre-existing phosphorylation.
      Sumoylated with SUMO1.
      Controlled by regulated intramembrane proteolysis (RIP). Undergoes metalloprotease-dependent constitutive ectodomain shedding to produce a membrane-anchored 52 kDa C-Terminal fragment which is further processed by presenilin gamma-secretase to yield an intracellular 50 kDa fragment.
    • 细胞定位

      Cell membrane.
    • Target information above from: UniProt accession P08069 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3480 Human
      • Entrez Gene: 16001 Mouse
      • Entrez Gene: 25718 Rat
      • Omim: 147370 Human
      • SwissProt: P08069 Human
      • SwissProt: Q60751 Mouse
      • SwissProt: P24062 Rat
      • Unigene: 643120 Human
      • Unigene: 714012 Human
      • Unigene: 275742 Mouse
      • Unigene: 10957 Rat
      • Unigene: 165078 Rat
      see all
    • 别名

      • CD221 antibody
      • CD221 antigen antibody
      • IGF 1 receptor antibody
      • IGF 1R antibody
      • IGF I receptor antibody
      • IGF-I receptor antibody
      • Igf1r antibody
      • IGF1R_HUMAN antibody
      • IGFIR antibody
      • IGFIRC antibody
      • IGFR antibody
      • Insulin like growth factor 1 receptor antibody
      • Insulin like growth factor 1 receptor precursor antibody
      • Insulin-like growth factor 1 receptor beta chain antibody
      • Insulin-like growth factor I receptor antibody
      • JTK13 antibody
      • MGC142170 antibody
      • MGC142172 antibody
      • MGC18216 antibody
      • Soluble IGF1R variant 1 antibody
      • Soluble IGF1R variant 2 antibody
      see all

    图片

    • Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)
      Western blot - Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681)
      All lanes : Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody (ab5681) at 1/1000 dilution (2 hours at room temperature in a 3% BSA-TBST buffer)

      Lane 1 : Unstimulated (-), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C
      Lanes 2-8 : Insulin stimulated (+), CHO-T transfected with insulin receptor containing vector whole cell extract with 5% BSA TBST buffer overnight at 4°C

      Secondary
      All lanes : goat F(ab’ 2 anti-rabbit IgG HRP conjugate


      Peptide Competition - prior primary antibody incubation:

      1 and 2 - no peptide, 

      3 - non-phosphorylated peptide corresponding to the immunogen,  

      4 -  generic phosphotyrosine-containing peptide,

      5 to 7 - phosphopeptides corresponding to other IR/IGF1R sites,

      8 - phosphopeptide immunogen.

      10% SDS-PAGE transferred to PVDF.

      Method of detection: Pierce SuperSignal method.

      The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpYpY1158/1162/1163] completely blocks the antibody signal, thereby demonstrating the specificty.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (9)

    发表研究结果有使用 ab5681?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5681 被引用在 9 文献中.

    • Huang TW  et al. Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2. Acta Biomater 97:399-408 (2019). PubMed: 31421230
    • Yang R  et al. Treatment of type 2 diabetes mellitus via reversing insulin resistance and regulating lipid homeostasis in vitro and in vivo using cajanonic acid A. Int J Mol Med 42:2329-2342 (2018). PubMed: 30226559
    • Allodi I  et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep 6:25960 (2016). PubMed: 27180807
    • Khan I  et al. Epithelial atrophy in oral submucous fibrosis is mediated by copper (II) and arecoline of areca nut. J Cell Mol Med 19:2397-412 (2015). WB . PubMed: 26248978
    • Hansen BF  et al. Molecular Characterisation of Long-Acting Insulin Analogues in Comparison with Human Insulin, IGF-1 and Insulin X10. PLoS One 7:e34274 (2012). PubMed: 22590494
    • Ammoun S  et al. The role of insulin-like growth factors signaling in merlin-deficient human schwannomas. Glia 60:1721-33 (2012). WB ; Human . PubMed: 22821509
    • Bouchentouf M  et al. Monocyte derivatives promote angiogenesis and myocyte survival in a model of myocardial infarction. Cell Transplant 19:369-86 (2010). PubMed: 20021736
    • Tanaka J  et al. Foxo1 links hyperglycemia to LDL oxidation and endothelial nitric oxide synthase dysfunction in vascular endothelial cells. Diabetes 58:2344-54 (2009). WB ; Human . PubMed: 19584310
    • Bobulescu IA  et al. Effect of renal lipid accumulation on proximal tubule Na+/H+ exchange and ammonium secretion. Am J Physiol Renal Physiol 294:F1315-22 (2008). PubMed: 18417539

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (placenta)
    Loading amount
    75 µg
    Specification
    placenta
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 07 2009

    Question

    I use three different anti-insulin receptor antibodies against different pho
    sphorylation sites (ab 60946 - phospho Y1361, ab5678 - phosoho Y972 and ab78355 - phospho Y1158. Appr
    oximately two years ago a phospho insulin receptor antibody was offered which recognized the sites Y1158, Y1162, Y1163. Sinc
    e I am interested in phospho sites Y1162, Y1163 of the insulin receptor I wonder if you offer such an antibody? Is the ab5681 anti IGF1receptor Y1158, Y1162, Y1163 specific for the IGF1 or does that antibody also recognize the phosphorylated insulin receptor at site Y1162 and Y1163?
    Thanks in advance.
    Sincerely,
    Janine Krüger

    Read More

    Abcam community

    Verified customer

    Asked on Aug 29 2012

    Answer

    Wenn Sie einen Antikörper suchen ,der nur mit dem IGF1 Rezeptor reagiert , wenn dieser an Y1158 + Y1162 + Y1163 phosphoryliert ist, ist dies der Richtige für Sie. Er reagiert nicht mit dem unmodifizierten Protein, und laut unserem Datenblatt reagiert der Antikörper nur mit dem Protein, wenn es eben an allen drei Phosphorylierungsstellen modifiziert ist.

    Read More

    Abcam Scientific Support

    回复于 Aug 29 2012

    Question

    i'm interested in detecting insulin receptor activation and have been looking at the literature. It is stablished that autophosphorylation of InsR,to undergo activation, takes place at tyr 1146, tyr 1150 and tyr1151. My question is ,why this phosphospecific Ab recognize phorylation at site 1158, 1162 and 1163? is it because the sequence is in different species? arr those sites known to be phosphorylated aswell, when binding of insulin to the receptor? thanks in advance for your time.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 12 2005

    Answer

    We have just received the following information from the source of ab5681: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR) as numbered according to Ebina, et al. (1146, 1150 and 1151 according to Ullrich, et al.). The corresponding residues in the IGF1R are 1131, 1135 and 1136. The sequence is conserved in human, mouse and rat for both the IR and IGF1R.The two relevant papers are:Ebina, Y., et al. (1985) The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40(4):747-758.Ullrich, A., et al. (1985) Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313(6005):756-761.When I look in NCBI, I see that there is a long and a short isoform of this protein. This is why we are listing 1158, 1162 and 1163 in the name (where these phospho sites in the long isoform) as well as 1146, 1150 and 1151 (for the short isoform) within the TDS. I hope this information helps, please do not hesitate to contact us again if you require further assistance,

    Read More

    Abcam Scientific Support

    回复于 Apr 14 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2022 艾博抗(上海)贸易有限公司版权所有