概述

  • 产品名称
  • 描述
    山羊多克隆抗体to IGF1
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • 免疫原

    Peptide with sequence

    C-RSVRAQRHTD

    , from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1.

  • 阳性对照
    • Human uterus lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa

性能

应用

Our Abpromise guarantee covers the use of ab106836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 疾病相关
    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 序列相似性
    Belongs to the insulin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • 别名
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

图片

  • Anti-IGF1 antibody (ab106836) at 0.5 µg/ml + Human uterus lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa

  • ab106836 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106836 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Eleftheriadou I  et al. aCAR IGF-1 vector targeting of motor neurons ameliorates disease progression in ALS mice. Ann Clin Transl Neurol 3:752-768 (2016). WB ; Human . Read more (PubMed: 27752511) »
  • Duran JM  et al. Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms. Circ Res 113:539-52 (2013). WB ; Mouse . Read more (PubMed: 23801066) »
See all 2 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Sample
Zebrafish Tissue lysate - whole (muslce (igf1 overexpression in transgenic fish mus)
Specification
muslce (igf1 overexpression in transgenic fish mus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Dr. Dongliang Li

Verified customer

提交于 Nov 28 2013

Answer

Merci de votre intérêt pour les anti-IGF1 de chez Abcam.

Aucun des anticorps anti-IGF1 Abcam n’a encore été testé à la fois chez l'homme, la souris et le rat et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab106836 par exemple (www.abcam.com/ab106836), il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un avoir de la valeur de l'anticorps testé vous sera offert.

Etapes à suivre :

1. Nous confirmer que vous souhaitez tester ab106836 chez le rat et/ou la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab106836

2. Acheter ab106836 par téléphone, fax ou internet (www.abcam.com)

3. Le tester chez le rat et/ou la souris

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab106836 ne pas fonctionne pas chez les espèces testées, après soumission de votre Abreview, vous recevrez quand même un avoir de la valeur de l'anticorps testé.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. We can recomend using Tris triton or RIPA buffer for lysates preparation. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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