Anti-IgA Secretory Component抗体[SC-05] (ab3924)

Reviews (1)Q&A (9)References (7)

Key features and details

  • Mouse monoclonal [SC-05] to IgA Secretory Component
  • Suitable for: IHC-Fr, IP, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-IgA Secretory Component抗体[SC-05]
    参阅全部 IgA Secretory Component 一抗

  • 描述

    小鼠单克隆抗体[SC-05] to IgA Secretory Component

  • 宿主

    Mouse

  • 特异性

    ab3924 reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). The recognized glycoprotein is a specific membrane marker of glandular carcinomas.

  • 经测试应用

    适用于: IHC-Fr, IP, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human IgA Secretory Component. Affinity-purified secretory component from human colostrum.

  • 阳性对照

    • Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

  • 常规说明


    Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • Primary antibody说明

    Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

  • 克隆

    单克隆

  • 克隆编号

    SC-05

  • 同种型

    IgG1

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). See Abreview. Use under non-reducing conditions.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性

    IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA). The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. Secretory component is a proteolytic cleavage product of the polymeric immunoglobulin receptor which remains associated with dimeric IgA in sero-mucus secretions. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. Secretory components wrap around two IgA units joined by a J chain protein fragment, resulting in a >--< configuration, with each of the two antigen binding regions of the two constituent y-shaped antibodies exposed. One identified function of secretory components is to protect IgA antibodies from degradation by the gastric acids and enzymes of the digestive system. This property is especially important in the transfer of immune system components during breastfeeding.

图片

  • Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
    Western blot - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
    All lanes : Anti-IgA Secretory Component antibody [SC-05] (ab3924) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Secretory Component Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunohistochemistry (Frozen sections) - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
    Immunohistochemistry (Frozen sections) - Anti-IgA Secretory Component antibody [SC-05] (ab3924)Image courtesy of an anonymous Abreview.

    ab3924 staining IgA Secretory Component in human normal breast tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab3924 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.The images show stainings of normal breast glands from two different patients. The antibody mostly stains the apical/luminal region of luminal epithelial cells, but the intensity varies from patient to patient.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA Secretory Component antibody [SC-05] (ab3924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA Secretory Component antibody [SC-05] (ab3924)Image from Wu QW et al., BMC Cancer. 2012 Jan 27;12:47. doi: 10.1186/1471-2407-12-47. Fig 2.; doi:10.1186/1471-2407-12-47; 27 January 2012, BMC Cancer 2012, 12:47

    Immunohistochemical analysis of Human breast cancer tissue, staining IgA Secretory Component with ab3924.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Tissue was blocked with 10% normal goat serum before incubating with primary antibody (1/50) for 2 hours at room temperature. Staining was primarily in the cytoplasm.

文献 (7)

发表研究结果有使用 ab3924？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3924 被引用在 7 文献中.

  • Heinbockel L  et al. Identification of novel target genes in human lung tissue involved in chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis 13:2255-2259 (2018). PubMed: 30100715
  • Wu Q  et al. Extracellular matrix protein 1 is correlated to carcinogenesis and lymphatic metastasis of human gastric cancer. World J Surg Oncol 12:132 (2014). IHC-P ; Human . PubMed: 24779890
  • Wu QW  et al. Expression and clinical significance of extracellular matrix protein 1 and vascular endothelial growth factor-C in lymphatic metastasis of human breast cancer. BMC Cancer 12:47 (2012). IHC-P ; Human . PubMed: 22284579
  • Polosukhin VV  et al. Bronchial secretory immunoglobulin a deficiency correlates with airway inflammation and progression of chronic obstructive pulmonary disease. Am J Respir Crit Care Med 184:317-27 (2011). IHC-P ; Human . PubMed: 21512171
  • Bartek J  et al. Keratin 19 expression in the adult and developing human mammary gland. Histochem J 22:537-44 (1990). PubMed: 1705251
  • B&#225;rtek J  et al. Secretory component in differentiating normal epithelium, benign lesions and malignancy in the human breast as monitored by monoclonal antibodies. Histochemistry 91:235-44 (1989). PubMed: 2722565
  • Kvale D  et al. Rapid selection of cultured cells with increased expression of a membrane marker (secretory component). Int J Cancer 42:638-41 (1988). PubMed: 2459074

客户评价及客户问答

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1-10 of 10 Abreviews or Q&A

Question

Do you have specific antibodies recognizing ECM1a and ECM1b independently working in WB or ELISA ?

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Answer

I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

The difference between these two isoforms is that ECM1b lacks amino acids 237-361. Therefore, if you had an antibody targeting this region, it would detect ECM1a but not ECM1b. You however wouldn't be able to specifically detect ECM1b as all the amino acids present in this isoform are also present in ECM1a.

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Question

I still think though that your datasheet is incorrect, because extracellular matrix protein 1 is not part of secretory IgA. Rather, the following protein (a proteolytic fragment of the poly-Ig receptor) is part of secretory IgA: http://www.uniprot.org/uniprot/P01833

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Answer

It appears that there is a nomenclature difference between your target of interest (secretory component) and our antibodies that are anti "secretory component glycoprotein" which is also referred to as "Extracellular matrix protein 1" and "secretory component p85"
Thus, it seems an anti-PIGR antibody would be more applicable for your needs. Even though an anti-PIGR antibody that only detects the cleaved secretory component without detecting uncleaved PIGR won't exist, we can find one that detects both cleaved secretory component and the uncleaved, full-length PIGR by choosing an antibody that recognizes aa 19-603 of PIGR. Our rabbit polyclonal anti-PIGR antibody that meets this requirement is listed below:
ab96196
https://www.abcam.com/pigr-antibody-ab96196.html

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Question

I've realized that all the info listed under "target", including all the links to databases are wrong and are not related to secretory component.

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Answer

For ab3924, the links to the databases and target information mention "Extracellular matrix protein 1" which is an alternative name for "secretory component p85" and "secretory component glycoprotein" according to the uniprot file below:
http://www.uniprot.org/uniprot/Q16610

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Question

Do you synthesize the anti-sera based on DNA sequence of proteins, if you do not, can you suggest me a company they make anti-sera, many thanks!

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Answer

Thank you for contacting us.

In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), some against protein fragments and others against the full length protein (either purified or overexpressed in another organism) or a cell preparation.

Which specific antibody or protein target do you have in mind?

As for the anti-IgA antibodies - the immunogendepends on the antibody.

Please let me know more details about the products of your interest and I'd be happy to assist you further.

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Question


Thanks for your answer,



In our Laboratory we study the expression and level of different molecules through immunofluorescense, it mean we have a significant number of primary and secondary antibodies and that I have tried different ways to make the Nodal ab55676 works without success. Additionally this antibody doesn't work in our system in vitro. I'm asking you for a sample of the Anti-Nodal antibody (ab39242) because we trust in Abcam products and I'm almost sure that the ab39242 will work better so, Is possible to get a sample of this antibody? Please, just take your time to answer my question, don't waste your time sending me others links.

Thanks,

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Answer

Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

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Question

Hello,



Recently I bought the Mouse monoclonal antibody ab55676. I have tried this antibody several times in a mouse model to study the role that Nodal could play in teramota incidence, a kind of cancer. Until now I don't have any good result because this antibody show a lot of unspecific binding. I'm writing to you with the hope to get a sample of the Anti-Nodal antibody ab3924 which is rabbit, and I think could work better in my samples..





I will be waiting for your answer,

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Answer

Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High background is a very common issue when working in tissues of the same species as the host speciesof the primary anti-body. These may be reduced by using a secondary antibody that has been pre-absorbed or by direct conjugation of your label to the primary antibody.

Our EasyLink conjugation kits offer an effective and simple way to directly conjugate labels to your primary antibody. More information may be found at the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

We have 12 secondary antibodies against mouse IgG1, they may be viewed at the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG1&pt=7&sk=targsp&sv=2&sn=Mouse&l=2&fViewMore=1

Please let me know if you have any questions.

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Immunohistochemistry (Frozen sections) abreview for Anti-Secretory Component Glycoprotein antibody [SC-05]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Normal breast)
Specification
Normal breast
Fixative
Methanol
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jan 04 2012

Question

Dear Technical team, Did you do epitope mapping for this antibody? The customer asked the epitope information for this antibody.Which part of protein does this antibody recognize? Please let us know. Thank you very much!

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Answer

Thank you for contacting us. Unfortunately we do not have any specific information about the epitope of Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924). We do however know that the epitope recognised is reduction-resistant and present in both the free and Ig-bound secretory component. More precise information regarding the epitope is not known.   I hope this information has been of help. If you need any further information please let us know and we will try to assist you. 

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Question

Does this antibody recognize the protein when it is part of the Polymeric Ig Receptor complex, or only when it is cleaved and secreted?

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Answer

Thank you for your enquiry. Unfortunately the only information the originator of the antibody was able to provide was mostly on the datasheet: The antibody reacts with 80 kDa human secretory component glycoprotein (both free and bound in secretory IgA). Recognized glycoprotein is specific membrane marker of glandular carcinomas. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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Question

I need to know the working dilution for IHC and Western blot.

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Answer

Thank you for your enquiry. There is no information currently available about the working dilution for this antibody in IHC and Western blot. For a purified antibody, we recommend starting dilutions for Western blot to be 1 ug/mL and for IHC, 10 ug/mL. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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