产品名称Anti-IFN gamma Receptor beta / AF-1抗体
参阅全部 IFN gamma Receptor beta / AF-1 一抗
描述兔多克隆抗体to IFN gamma Receptor beta / AF-1
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Human, Pig
预测可用于: Cow, Baboon, Deer
Synthetic peptide corresponding to Human IFN gamma Receptor beta/ AF-1 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Tissues: Human spinal cord; Mouse liver; Human skeletal muscle; Mouse kidney. Whole cell: Hela nuclear; F9; A431
Previously labelled as IFN gamma Receptor beta.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab77246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).|
功能Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
疾病相关Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
序列相似性Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
- Information by UniProt
- AF 1 antibody
- AF-1 antibody
- AF1 antibody
All lanes : Anti-IFN gamma Receptor beta / AF-1 antibody (ab77246) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : F9 (Mouse embryonic carcinoma cell line)
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 7 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 48 kDa (possible cleavage fragment), 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Interferon-gamma receptor beta chain has a predicted molecular weight of 38 kDa, however it is extensively glycosylated and has been shown to run in the 49 kDa region (SwissProt data). The bands present at 48 kDa are representative of the protein minus its 27 amino acid signal peptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This product has been referenced in:
- Zhang S et al. Swiprosin-1 deficiency impairs macrophage immune response of septic mice. JCI Insight 3:N/A (2018). Read more (PubMed: 29415882) »
- Ma X et al. Alendronate promotes osteoblast differentiation and bone formation in ovariectomy-induced osteoporosis through interferon-ß/signal transducer and activator of transcription 1 pathway. Exp Ther Med 15:182-190 (2018). Read more (PubMed: 29375681) »