概述

  • 产品名称
  • 描述
    兔多克隆抗体to IDOL
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human IDOL aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab126642)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.

性能

应用

Our Abpromise guarantee covers the use of ab107168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • 组织特异性
    Ubiquitously expressed.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • 发展阶段
    Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • 结构域
    The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • 翻译后修饰
    Autoubiquitinated.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BM-023 antibody
    • BZF1 antibody
    • E3 ubiquitin protein ligase MYLIP antibody
    • E3 ubiquitin-protein ligase MYLIP antibody
    • Idol antibody
    • Inducible degrader of the LDL-receptor antibody
    • Lipoprotein receptor antibody
    • MIR antibody
    • MYLIP antibody
    • MYLIP_HUMAN antibody
    • Myosin regulatory light chain interacting protein antibody
    • PP5242 antibody
    see all

图片

  • All lanes : Anti-IDOL antibody (ab107168) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 32 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

实验方案

文献

ab107168 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

Thank you for submitting an Abreview of ab107168. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

-loading more protein for the cell lysate samples

But your protocol looks fine, thus I am not sure why the antibody is not picking up the protein in the cell lysates.


This antibody is covered by our Abpromise, thusifthe antibody was purchased within the past 6 months, I would be happy to discuss a refund, credit or replacement with you to resolve this issue.

Please let me know your order or PO number in that regard.

I look forward to your reply.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Left ventricle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Left ventricle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Aug 29 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Left ventricle, liver, spleen, brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Left ventricle, liver, spleen, brain
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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