参阅全部 IDOL 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human IDOL aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; SHSY5Y; NIH3T3.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度Immunogen affinity purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab107168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).|
功能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
通路Protein modification; protein ubiquitination.
序列相似性Contains 1 FERM domain.
Contains 1 RING-type zinc finger.
发展阶段Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
结构域The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
- Information by UniProt
- BM-023 antibody
- BZF1 antibody
- E3 ubiquitin protein ligase MYLIP antibody
All lanes : Anti-IDOL antibody (ab107168) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
ab107168 has not yet been referenced specifically in any publications.