使用敲除细胞株进行验证重组RabMAb

重组Anti-IDH1抗体[EPR12296] (ab172964)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12296] to IDH1
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-IDH1抗体[EPR12296]
    参阅全部 IDH1 一抗

  • 描述

    兔单克隆抗体[EPR12296] to IDH1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Cow, Orangutan

  • 免疫原

    Synthetic peptide within Human IDH1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75874

  • 阳性对照

    • WB: HAP1, HepG2, HeLa, and Raji cell lysates and human fetal kidney tissue lysate. IHC-P: Human breast carcinoma tissue. IP: HepG2 cell lysate.

  • 常规说明

    This product was previously labelled as Isocitrate dehydrogenase.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab172964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt.

    • 靶标

    • 疾病相关

      Glioma
      Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

    • 序列相似性

      Belongs to the isocitrate and isopropylmalate dehydrogenases family.

    • 翻译后修饰

      Acetylation at Lys-374 dramatically reduces catalytic activity.

    • 细胞定位

      Cytoplasm. Peroxisome.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Cytosolic NADP isocitrate dehydrogenase antibody
      • Cytosolic NADP-isocitrate dehydrogenase antibody
      • Epididymis luminal protein 216 antibody
      • Epididymis secretory protein Li 26 antibody
      • HEL-216 antibody
      • HEL-S-26 antibody
      • ICDH antibody
      • IDCD antibody
      • IDH antibody
      • IDH1 antibody
      • IDHC_HUMAN antibody
      • IDP antibody
      • IDPC antibody
      • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
      • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
      • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
      • NADP dependent isocitrate dehydrogenase cytosolic antibody
      • NADP dependent isocitrate dehydrogenase peroxisomal antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      • PICD antibody
      see all

    图片

    • Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)
      Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)
      Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)
      All lanes : Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : HeLa lysate
      Lane 3 : Raji lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling IDH1 with ab172964 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)
      Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)

      Western blot analysis on immunoprecipitation pellet from (1) HepG2 cell lysate or (2) 1X PBS (negative control) using ab172964 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-IDH1 antibody [EPR12296] (ab172964)
      Anti-IDH1 antibody [EPR12296] (ab172964)

    文献 (7)

    发表研究结果有使用 ab172964？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab172964 被引用在 7 文献中.

    • Pillai-Kastoori L  et al. Antibody validation for Western blot: By the user, for the user. J Biol Chem 295:926-939 (2020). PubMed: 31819006
    • Shi F  et al. Wild-type IDH2 contributes to Epstein-Barr virus-dependent metabolic alterations and tumorigenesis. Mol Metab 36:100966 (2020). PubMed: 32224436
    • Nie Y  et al. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J 33:10705-10716 (2019). PubMed: 31262191
    • Liu Z  et al. High expression of PIM2 induces HSC proliferation in myelodysplastic syndromes via the IDH1/HIF1-a signaling pathway. Oncol Lett 17:5395-5402 (2019). PubMed: 31186757
    • Zhang W  et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856
    • Costa Nunes F  et al. Tacrine derivatives stimulate human glioma SF295 cell death and alter important proteins related to disease development: An old drug for new targets. Biochim Biophys Acta 1862:1527-1536 (2018). PubMed: 29704527
    • Ma S  et al. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget 6:8606-20 (2015). PubMed: 25825982

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-IDH1 antibody [EPR12296]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    Yes - 50% EtOH
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 23 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IDH1 antibody [EPR12296]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549 Lung cancer cells)
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
    Specification
    A549 Lung cancer cells
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 Mar 11 2015

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