Application
Flow Cytometry
Sample
Human Cell (aortic endothelial cells)
Specification
aortic endothelial cells
Preparation
Cell harvesting/tissue preparation method: mild trypsinisation
Sample buffer: pbs
Sample buffer: pbs
Fixation
Paraformaldehyde
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: pbs
Secondary antibody
Name
Non-Abcam antibody was used: Goat ant--mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/20
Additional data
Additional Notes
Graph 1 - control cells
Graph 2 - cells treated with TNFalpha for 4 hours
Graph 2 - cells treated with TNFalpha for 4 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 22 2008