Anti-ICAM1抗体[MEM-111] (ab2213)


  • 产品名称
    参阅全部 ICAM1 一抗
  • 描述
    小鼠单克隆抗体[MEM-111] to ICAM1
  • 宿主
  • 特异性
    This antibody reacts with human CD54 antigen.
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Flow Cyt, Sandwich ELISAmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).

  • 阳性对照
    • For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control. Sandwich ELISA: supernatant of HUVEC cell line. Indirect ELISA: endothelial cell line derived from bovine pulmonary artery. RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.



Our Abpromise guarantee covers the use of ab2213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
ELISA Use a concentration of 25 µg/ml. Use at a dilution of 1/1000 for Indirect ELISA, 1hr, RT. The antibody reacts with tissue - endothelial cell line derived from bovine pulmonary artery.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to ICAM1 (Biotin) (ab7815).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.


  • 功能
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all


  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)

    Lane 1 : TNF-alpha activated HUVEC cells
    Lane 2 : TNF-alpha nonactivated HUVEC cells

    Performed under non-reducing conditions.

    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

    Lower bands represent tubulin as a loading control.

  • Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
  • ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

    See Abreview

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution

    Lane 1 : Western Markers.
    Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
    Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.

    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    Observed band size: 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa (possible non-specific binding)

    See Abreview


This product has been referenced in:
  • Li Y  et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med 41:2629-2639 (2018). Read more (PubMed: 29484379) »
  • Kotha S  et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One 13:e0195082 (2018). ICC/IF . Read more (PubMed: 29617409) »

See all 19 Publications for this product


I sorry to hear about the volume of the antibody; we always fill vials 4-5ul extra so quick spin is required to extract the whole volume. I am sure you have given a quick spin to vial before opening it, if it hasn't been done then it will worth to try....

Read More

Thank you for your enquiry.

As the Swiss-Prot database highlights, the mature ICAM1 protein is heavily N-Glycosylated. There at least 9 potential site for this type of post-translational modification which can significantly alter the band siz...

Read More

Thank you for your response.

Could you please specify the size of the non-specific bands? Are they lower or higher than the expected size (58 kDa)? Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at t...

Read More

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

Read More

Thank you for calling us yesterday and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed...

Read More

I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.


Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

Read More

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact o...

Read More

Thank you for contacting Abcam regarding ab2213.

I am sorry that you have been experiencing difficulities with this antibody in IHC. Based on the information you provided I agree that this product is not performing as stated on the datasheet. ...

Read More

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

Read More

Thank you for your reply.

The options of resolving this case are to replace the antibody free of charge with another antibody (different lot; same target; different target)or to issue a credit or refund.

I assume from the email con...

Read More

1-10 of 23 Q&A