Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).
For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.
Sandwich ELISA: supernatant of HUVEC cell line.
Indirect ELISA: endothelial cell line derived from bovine pulmonary artery.
RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Flow Cytometry - Anti-ICAM1 antibody [MEM-111] (ab2213)This image is courtesy of an Abreview submitted by Dr Karppiah Muthumani
ab2213 staining human PBL cells by flow cytometry. Cells were formaldehyde fixed and gated on B-cells. The primary antibody was incubated with the sample for 30 minutes at 25°C. The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L). Both antibodies were used undiluted.
Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)This image is courtesy of an anonymous Abreview
All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution
Lane 1 : Western Markers. Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells. Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.
Secondary All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
Li Y et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med41:2629-2639 (2018).
Read more (PubMed: 29484379) »
Kotha S et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One13:e0195082 (2018).
Read more (PubMed: 29617409) »