概述

  • 产品名称
    Anti-ICAM1抗体[MEM-111]
    参阅全部 ICAM1 一抗
  • 描述
    小鼠单克隆抗体[MEM-111] to ICAM1
  • 宿主
    Mouse
  • 特异性
    This antibody reacts with human CD54 antigen.
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Flow Cyt, Sandwich ELISAmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).

  • 阳性对照
    • For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control. Sandwich ELISA: supernatant of HUVEC cell line. Indirect ELISA: endothelial cell line derived from bovine pulmonary artery. RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.

性能

应用

Our Abpromise guarantee covers the use of ab2213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
ELISA Use a concentration of 25 µg/ml. Use at a dilution of 1/1000 for Indirect ELISA, 1hr, RT. The antibody reacts with tissue - endothelial cell line derived from bovine pulmonary artery.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to ICAM1 (Biotin) (ab7815).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.

靶标

  • 功能
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

图片

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)

    Lane 1 : TNF-alpha activated HUVEC cells
    Lane 2 : TNF-alpha nonactivated HUVEC cells

    Performed under non-reducing conditions.

    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Lower bands represent tubulin as a loading control.

  • Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
  • ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

    See Abreview

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution

    Lane 1 : Western Markers.
    Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
    Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    Observed band size: 95 kDa why is the actual band size different from the predicted?
    Additional bands at: 40 kDa (possible non-specific binding)

    See Abreview

文献

This product has been referenced in:
  • Li Y  et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med 41:2629-2639 (2018). Read more (PubMed: 29484379) »
  • Kotha S  et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One 13:e0195082 (2018). ICC/IF . Read more (PubMed: 29617409) »
See all 23 Publications for this product

客户评价及客户问答

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1-10 of 12 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Blocking
Sample
Streptococcus pneumoniae Cell (Primary Bronchial Epithelial cells)
Specification
Primary Bronchial Epithelial cells

Dr. Selvarani Vimalanathan

Verified customer

提交于 Aug 03 2018

Application
Immunocytochemistry
Sample
Human Cultured Cells (BEAS-2B Epithelial cells)
Permeabilization
Yes - 0.5% saponin
Specification
BEAS-2B Epithelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Selvarani Vimalanathan

Verified customer

提交于 Aug 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines total lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Cancer cell lines total lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 07 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Rhesus monkey Cell lysate - whole cell (RF/6A cells from ATCC)
Specification
RF/6A cells from ATCC
Treatment
5.5 mM glucose, 30 mM glucose, 30 mM glucose + 0.1 mM BAPN for 10 days
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (rat cortex (injured))
Specification
rat cortex (injured)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X-100 in blocking buffer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Mar 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cell)
Specification
Mesenchymal stem cell
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Mr. Steven Johnstone

Verified customer

提交于 Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung, appendix)
Specification
lung, appendix
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

提交于 Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
100000 cells
Specification
HUVEC
Treatment
1 ng/ml TNF-alpha for 24 h
Gel Running Conditions
Non-reduced Denaturing (10 % gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Aug 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBL)
Specification
PBL
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
B- cell

Dr. Karppiah Muthumani

Verified customer

提交于 May 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (aortic endothelial cells)
Specification
aortic endothelial cells
Preparation
Cell harvesting/tissue preparation method: mild trypsinisation
Sample buffer: pbs
Fixation
Paraformaldehyde
Permeabilization
No

Abcam user community

Verified customer

提交于 Apr 22 2008

1-10 of 12 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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