Anti-ICAM1抗体[MEM-111] (ab2213)

概述

  • 产品名称
    Anti-ICAM1抗体[MEM-111]
    参阅全部 ICAM1 一抗
  • 描述
    小鼠单克隆抗体[MEM-111] to ICAM1
  • 宿主
    Mouse
  • 特异性
    This antibody reacts with human CD54 antigen.
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Flow Cyt, Sandwich ELISAmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).

  • 阳性对照
    • For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control. Sandwich ELISA: supernatant of HUVEC cell line. Indirect ELISA: endothelial cell line derived from bovine pulmonary artery. RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.

性能

应用

Our Abpromise guarantee covers the use of ab2213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
ELISA Use a concentration of 25 µg/ml. Use at a dilution of 1/1000 for Indirect ELISA, 1hr, RT. The antibody reacts with tissue - endothelial cell line derived from bovine pulmonary artery.
WB Use at an assay dependent concentration. Use under non-reducing conditions.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to ICAM1 (Biotin) (ab7815).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.

靶标

  • 功能
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

图片

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)

    Lane 1 : TNF-alpha activated HUVEC cells
    Lane 2 : TNF-alpha nonactivated HUVEC cells

    Performed under non-reducing conditions.

    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Lower bands represent tubulin as a loading control.

  • Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
  • ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

    See Abreview

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution

    Lane 1 : Western Markers.
    Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
    Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    Observed band size: 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa (possible non-specific binding)

    See Abreview

文献

This product has been referenced in:
  • Li Y  et al. Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment. Int J Mol Med 41:2629-2639 (2018). Read more (PubMed: 29484379) »
  • Kotha S  et al. Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment. PLoS One 13:e0195082 (2018). ICC/IF . Read more (PubMed: 29617409) »

See all 19 Publications for this product

客户评价及客户问答

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Application
Immunocytochemistry
Sample
Human Cultured Cells (BEAS-2B Epithelial cells)
Permeabilization
Yes - 0.5% saponin
Specification
BEAS-2B Epithelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Selvarani Vimalanathan

Verified customer

提交于 Aug 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines total lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Cancer cell lines total lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 07 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Rhesus monkey Cell lysate - whole cell (RF/6A cells from ATCC)
Specification
RF/6A cells from ATCC
Treatment
5.5 mM glucose, 30 mM glucose, 30 mM glucose + 0.1 mM BAPN for 10 days
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (rat cortex (injured))
Specification
rat cortex (injured)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X-100 in blocking buffer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cell)
Specification
Mesenchymal stem cell
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Steven Johnstone

Verified customer

提交于 Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung, appendix)
Specification
lung, appendix
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 20 min, 100C
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
100000 cells
Specification
HUVEC
Treatment
1 ng/ml TNF-alpha for 24 h
Gel Running Conditions
Non-reduced Denaturing (10 % gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBL)
Specification
PBL
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
B- cell
Username

Dr. Karppiah Muthumani

Verified customer

提交于 May 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (aortic endothelial cells)
Specification
aortic endothelial cells
Preparation
Cell harvesting/tissue preparation method: mild trypsinisation
Sample buffer: pbs
Fixation
Paraformaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (HUVEC)
Specification
HUVEC
Preparation
Cell harvesting/tissue preparation method: trypsinised from flask
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
unstained HUVEC
Username

Abcam user community

Verified customer

提交于 Feb 05 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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