Anti-ICAM1抗体[15.2] (ab20)

Reviews (1)Q&A (2)References (21)

概述

  • 产品名称

    Anti-ICAM1抗体[15.2]
    参阅全部 ICAM1 一抗

  • 描述

    小鼠单克隆抗体[15.2] to ICAM1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, Inhibition Assay, Flow Cyt, IHC-Fr, IPmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Human monocytes.

  • 阳性对照

    • WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.

性能

应用

Our Abpromise guarantee covers the use of ab20 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
Inhibition Assay Use at an assay dependent concentration. PubMed: 23825944
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC-P.

    • 靶标

    • 功能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

    • 序列相似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻译后修饰

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      图片

      • Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

        Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
        Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
        Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 58 kDa
        Observed band size: 58 kDa

      • Immunohistochemistry (Frozen sections) - Anti-ICAM1 antibody [15.2] (ab20)
        Immunohistochemistry (Frozen sections) - Anti-ICAM1 antibody [15.2] (ab20)Allen et al. PLoS One. 2013 Oct 30;8(10):e78441. doi: 10.1371/journal.pone.0078441. eCollection 2013. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Expression of proteins with functions involved in ocular disease and wound healing in AM substrates.

        Proteins detected include growth factors and biomarkers (A) cell adhesion, cytokine and angiogenesis markers (B) metalloproteases and (C) neurotrophic factors (D). The collective pattern in staining demonstrates comparable levels of expression in trehalose and raffinose treated AM compared to fresh and increased expression compared to cryopreserved AM. Positive staining is represented as green or yellow and AEC nuclei were counterstained with DAPI (blue). Images shown are representative of triplicate experiments carried out on three donor membranes. Scale bar, 100 µm.

      • Flow Cytometry - Anti-ICAM1 antibody [15.2] (ab20)
        Flow Cytometry - Anti-ICAM1 antibody [15.2] (ab20)Morishita et al. PLoS One. 2011;6(8):e23267. doi: 10.1371/journal.pone.0023267. Epub 2011 Aug 5. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        ICAM-1 expression on HK-2 cells.

        HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0, 1.0 and 0.1 ng/ml) were stained with ab20 and isotype control mAb (A). HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0 ng/ml) were stained with ab20 and isotype control mAb at 24 hrs, 48 hrs and 72 hrs after TGF-β1 stimulation (B). Cells were analyzed by flow cytometry. Closed histogram: ICAM-1-stained cells. Dotted line: isotype control stained cells. MFI: mean fluorescence intensity.

      • Inhibition Assay - Anti-ICAM1 antibody [15.2] (ab20)
        Inhibition Assay - Anti-ICAM1 antibody [15.2] (ab20)Avril et al. PLoS Pathog. 2013;9(6):e1003430. doi: 10.1371/journal.ppat.1003430. Epub 2013 Jun 27. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Binding levels of P. falciparum-infected erythrocytes increase after endothelial cell selection.

      文献

      This product has been referenced in:

      • Xu L  et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). Read more (PubMed: 31354796) »
      • Bernabeu M  et al. Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1. mBio 10:N/A (2019). Read more (PubMed: 31138740) »
      See all 21 Publications for this product

      发表研究结果有使用 ab20？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      We have informed that the product ab27536, which was discontinued. We have been received the information given by you, but I contacted with the customer and she told me she need the antibody is able to recognize the ICAM1 protein intra- and extracellular in samples extracted from human. I would like you may confirm these antibodies recommended by you could work in this condition. Additionally, in the Abcam webpage, I found three antibodies, which are shown below: Anti-ICAM1 antibody [MEM-111] ab2213 Anti-ICAM1 antibody [YN1/1.7.4] ab25375 Anti-ICAM1 antibody (Biotin) ab7815 Which could these ones the differences with the others? Thanks for your support

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      Answer

      Thank you for the response.

      Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

      Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

      I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

      ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

      ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

      ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

      Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

      I hope this information is helpful. Please do not hesitate to contact me with further questions.

      Read More

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ICAM1 antibody [15.2]

       Poor
      Abreviews
      This product is known to not work in this application or species.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Urothelium, Bladder Cancer)
      Specification
      Urothelium, Bladder Cancer
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Read More

      Mr. Rudolf Jung

      Verified customer

      提交于 Aug 22 2006

      Question

      Are all antibodies 2952 - 2955 of the IgG1 subclass? This is important for us to chose the right secondary antibody - we are considering IgG1 PerCP from Becton Dickinson. Are you aware of a different red-shifted secondary antibody one could use with 488 laser excitation? Thanks a lot, kind regards, Ulf Steller Janssen Research Foundation Belgium

      Read More
      Answer

      Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com