Anti-ICAM1抗体[15.2] (ab20)
Key features and details
- Mouse monoclonal [15.2] to ICAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-ICAM1抗体[15.2]
参阅全部 ICAM1 一抗 -
描述
小鼠单克隆抗体[15.2] to ICAM1 -
宿主
Mouse -
Tested Applications & Species
Application Species WB Human -
免疫原
Human monocytes.
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阳性对照
- WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. -
克隆
单克隆 -
克隆编号
15.2 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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WB |
Human
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应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
靶标
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功能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
序列相似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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别名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
图片
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All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab20 被引用在 23 文献中.
- Xu L et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). PubMed: 31354796
- Bernabeu M et al. Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1. mBio 10:N/A (2019). PubMed: 31138740
- Wu D et al. The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy. Med Sci Monit 25:6946-6954 (2019). PubMed: 31860633
- Fu C et al. Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2-Nrf2 signaling pathway. Mol Med Rep 17:3877-3883 (2018). PubMed: 29359784
- Wang M et al. Intercellular adhesion molecule 1 antibody-mediated mesoporous drug delivery system for targeted treatment of triple-negative breast cancer. J Colloid Interface Sci 538:630-637 (2018). PubMed: 30554096