- 数据表
- 具体的参考文献 (13)
- 实验方案
概述
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产品名称Anti-ICAM1抗体[15.2]
参阅全部 ICAM1 一抗 -
描述小鼠单克隆抗体[15.2] to ICAM1
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宿主Mouse
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经测试应用适用于: WB, Inhibition Assay, Flow Cyt, IHC-Fr, IPmore details
不适用于: IHC-P -
种属反应性与反应: Human
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免疫原
Human monocytes.
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阳性对照
- WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
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存储溶液pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度Affinity purified
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纯化说明This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis.
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克隆单克隆
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克隆编号15.2
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骨髓瘤Sp2/0
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
应用
Our Abpromise guarantee covers the use of ab20 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. | |
| Inhibition Assay | Use at an assay dependent concentration. PubMed: 23825944 | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
靶标
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功能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
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序列相似性Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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别名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
图片
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![Immunohistochemistry (Frozen sections) - Anti-ICAM1 antibody [15.2] (ab20)](https://www.abcam.cn/ps/products/0/ab20/Images/ab20-300718-20999.jpg)
Immunohistochemistry (Frozen sections) - Anti-ICAM1 antibody [15.2] (ab20)Allen et al. PLoS One. 2013 Oct 30;8(10):e78441. doi: 10.1371/journal.pone.0078441. eCollection 2013. Fig 2.Expression of proteins with functions involved in ocular disease and wound healing in AM substrates.
Proteins detected include growth factors and biomarkers (A) cell adhesion, cytokine and angiogenesis markers (B) metalloproteases and (C) neurotrophic factors (D). The collective pattern in staining demonstrates comparable levels of expression in trehalose and raffinose treated AM compared to fresh and increased expression compared to cryopreserved AM. Positive staining is represented as green or yellow and AEC nuclei were counterstained with DAPI (blue). Images shown are representative of triplicate experiments carried out on three donor membranes. Scale bar, 100 µm.
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![Flow Cytometry - Anti-ICAM1 antibody [15.2] (ab20)](https://www.abcam.cn/ps/products/0/ab20/Images/ab20-300708-20666.jpg)
Flow Cytometry - Anti-ICAM1 antibody [15.2] (ab20)Morishita et al. PLoS One. 2011;6(8):e23267. doi: 10.1371/journal.pone.0023267. Epub 2011 Aug 5. Fig 1.ICAM-1 expression on HK-2 cells.
HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0, 1.0 and 0.1 ng/ml) were stained with ab20 and isotype control mAb (A). HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0 ng/ml) were stained with ab20 and isotype control mAb at 24 hrs, 48 hrs and 72 hrs after TGF-β1 stimulation (B). Cells were analyzed by flow cytometry. Closed histogram: ICAM-1-stained cells. Dotted line: isotype control stained cells. MFI: mean fluorescence intensity.
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![Western blot - Anti-ICAM1 antibody [15.2] (ab20)](https://www.abcam.cn/ps/products/0/ab20/Images/ICAM1-Primary-antibodies-ab20-2.jpg)
Western blot - Anti-ICAM1 antibody [15.2] (ab20)All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa -
![Inhibition Assay - Anti-ICAM1 antibody [15.2] (ab20)](https://www.abcam.cn/ps/products/0/ab20/Images/ab20-300712-20777.jpg)
Inhibition Assay - Anti-ICAM1 antibody [15.2] (ab20)Avril et al. PLoS Pathog. 2013;9(6):e1003430. doi: 10.1371/journal.ppat.1003430. Epub 2013 Jun 27. Fig S2.Binding levels of P. falciparum-infected erythrocytes increase after endothelial cell selection.
实验方案
文献
This product has been referenced in:
- van Balen P et al. Tissue Damage Caused by Myeloablative, but Not Non-Myeloablative, Conditioning before Allogeneic Stem Cell Transplantation Results in Dermal Macrophage Recruitment without Active T-Cell Interaction. Front Immunol 9:331 (2018). Read more (PubMed: 29535719) »
- Yasen A et al. Release of HIV-1 sequestered in the vesicles of oral and genital mucosal epithelial cells by epithelial-lymphocyte interaction. PLoS Pathog 13:e1006247 (2017). Read more (PubMed: 28241053) »
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