Anti-ICAM1抗体[15.2] (ab20)

Reviews (1)Q&A (2)References (25)
Western blot - Anti-ICAM1 antibody [15.2] (ab20)

    Key features and details

    • Mouse monoclonal [15.2] to ICAM1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    选择批间可重复性更高的重组抗体

    Product image
    Anti-ICAM1 antibody [EP1442Y] (ab53013)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-ICAM1抗体[15.2]
      参阅全部 ICAM1 一抗

    • 描述

      小鼠单克隆抗体[15.2] to ICAM1

    • 宿主

      Mouse

    • 经测试应用

      适用于: WBmore details
      不适用于: IHC-P

    • 种属反应性

      与反应: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human ICAM1. Human monocytes.

    • 阳性对照

      • WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab20于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
    说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
  • 应用说明
    Is unsuitable for IHC-P.

    • 靶标

    • 功能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

    • 序列相似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻译后修饰

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      图片

      • Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        Western blot - Anti-ICAM1 antibody [15.2] (ab20)
        All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

        Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
        Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
        Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 58 kDa
        Observed band size: 58 kDa

      文献 (25)

      发表研究结果有使用 ab20？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab20 被引用在 25 文献中.

      • Ma L  et al. Agrin Influences Botulinum Neurotoxin A-Induced Nerve Sprouting via miR-144-agrin-MuSK Signaling. Front Cell Dev Biol 8:15 (2020). PubMed: 32083076
      • Bernabeu M  et al. Binding Heterogeneity of Plasmodium falciparum to Engineered 3D Brain Microvessels Is Mediated by EPCR and ICAM-1. mBio 10:N/A (2019). PubMed: 31138740
      • Wu D  et al. The Effects of the CXCR4 Antagonist, AMD3465, on Human Retinal Vascular Endothelial Cells (hRVECs) in a High Glucose Model of Diabetic Retinopathy. Med Sci Monit 25:6946-6954 (2019). PubMed: 31860633
      • Iwasaki O  et al. Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization. Nat Commun 10:5688 (2019). PubMed: 31831736
      • Xu L  et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). PubMed: 31354796
      View all Publications for this product

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ICAM1 antibody [15.2]

       Poor
      Abreviews
      This product is known to not work in this application or species.
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Urothelium, Bladder Cancer)
      Specification
      Urothelium, Bladder Cancer
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Read More

      Mr. Rudolf Jung

      Verified customer

      提交于 Aug 22 2006

      Question

      We have informed that the product ab27536, which was discontinued. We have been received the information given by you, but I contacted with the customer and she told me she need the antibody is able to recognize the ICAM1 protein intra- and extracellular in samples extracted from human. I would like you may confirm these antibodies recommended by you could work in this condition. Additionally, in the Abcam webpage, I found three antibodies, which are shown below: Anti-ICAM1 antibody [MEM-111] ab2213 Anti-ICAM1 antibody [YN1/1.7.4] ab25375 Anti-ICAM1 antibody (Biotin) ab7815 Which could these ones the differences with the others? Thanks for your support

      Read More
      Answer

      Thank you for the response.

      Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

      Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

      I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

      ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

      ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

      ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

      Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

      I hope this information is helpful. Please do not hesitate to contact me with further questions.

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      Question

      Are all antibodies 2952 - 2955 of the IgG1 subclass? This is important for us to chose the right secondary antibody - we are considering IgG1 PerCP from Becton Dickinson. Are you aware of a different red-shifted secondary antibody one could use with 488 laser excitation? Thanks a lot, kind regards, Ulf Steller Janssen Research Foundation Belgium

      Read More
      Answer

      Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs.

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