概述

  • 产品名称

    Anti-ICAM1抗体[15.2]
    参阅全部 ICAM1 一抗
  • 描述

    小鼠单克隆抗体[15.2] to ICAM1
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, Inhibition Assay, Flow Cyt, IHC-Fr, IPmore details
    不适用于: IHC-P
  • 种属反应性

    与反应: Human
  • 免疫原

    Human monocytes.

  • 阳性对照

    • WB: SK N BE, Saos-2 and HT1080 whole cell lysate. Flow Cytometry: HK-2 cells stimulated with TGF-beta1. IHC: Human amniotic membrane tissues.

性能

应用

Our Abpromise guarantee covers the use of ab20 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
Inhibition Assay Use at an assay dependent concentration. PubMed: 23825944
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • 序列相似性

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻译后修饰

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • 细胞定位

      Membrane.
    • Information by UniProt
    • 数据库链接

    • 别名

      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    图片

    • All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml

      Lane 1 : SK N BE (Human neuroblastoma) whole cell lysate
      Lane 2 : Saos-2 (Human epithelial-like osteosarcoma cell line) whole cell lysate
      Lane 3 : HT1080 (Human fibrosarcoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Expression of proteins with functions involved in ocular disease and wound healing in AM substrates.

      Proteins detected include growth factors and biomarkers (A) cell adhesion, cytokine and angiogenesis markers (B) metalloproteases and (C) neurotrophic factors (D). The collective pattern in staining demonstrates comparable levels of expression in trehalose and raffinose treated AM compared to fresh and increased expression compared to cryopreserved AM. Positive staining is represented as green or yellow and AEC nuclei were counterstained with DAPI (blue). Images shown are representative of triplicate experiments carried out on three donor membranes. Scale bar, 100 µm.

    • ICAM-1 expression on HK-2 cells.

      HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0, 1.0 and 0.1 ng/ml) were stained with ab20 and isotype control mAb (A). HK-2 cells (Mock) and HK-2 cells stimulated with TGF-β1 (10.0 ng/ml) were stained with ab20 and isotype control mAb at 24 hrs, 48 hrs and 72 hrs after TGF-β1 stimulation (B). Cells were analyzed by flow cytometry. Closed histogram: ICAM-1-stained cells. Dotted line: isotype control stained cells. MFI: mean fluorescence intensity.

    • Binding levels of P. falciparum-infected erythrocytes increase after endothelial cell selection.

    文献

    This product has been referenced in:

    • van Balen P  et al. Tissue Damage Caused by Myeloablative, but Not Non-Myeloablative, Conditioning before Allogeneic Stem Cell Transplantation Results in Dermal Macrophage Recruitment without Active T-Cell Interaction. Front Immunol 9:331 (2018). Read more (PubMed: 29535719) »
    • Wang M  et al. Intercellular adhesion molecule 1 antibody-mediated mesoporous drug delivery system for targeted treatment of triple-negative breast cancer. J Colloid Interface Sci 538:630-637 (2018). Read more (PubMed: 30554096) »
    See all 18 Publications for this product

    客户评价及客户问答

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for the response.

    Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

    Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

    I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

    ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

    ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

    ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

    Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

    I hope this information is helpful. Please do not hesitate to contact me with further questions.

    Read More
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Urothelium, Bladder Cancer)
    Specification
    Urothelium, Bladder Cancer
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated

    Mr. Rudolf Jung

    Verified customer

    提交于 Aug 22 2006

    Answer

    Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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