Application
IHC - Wholemount
Sample
Mouse Tissue (Retinal Flat mount)
Specification
Retinal Flat mount
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Standard lab protocol
Concentration
1 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The identical protocol and treatment conditions were used for this successful staining as the previously submitted trials that were unsuccessful, confirming that this was an antibody issue and not a protocol optimization issue. I will attach a successful image of the staining for reference. Secondary was the same color as the unsuccessful trials as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Tyler Kilburn
Verified customer
提交于 Mar 23 2018