Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 23°C · Diluent: Vector Labs block and diluent
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Vector Lab ImmPRESS Anti-goat polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Fixed in 10% neutral buffered formalin at room temperature for 2 days. Retrieval done at 95C for 20 minutes. Several buffers worked but EDTA ph9 was best. Other tissues such as lung, liver and spleen stained very well also.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Victor Bernal
Verified customer
提交于 Aug 26 2020