Application
Western blot
Sample
Escherichia coli Purified protein (Purified protein)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
2 µg
Treatment
Protease cleavage 3hrs
Specification
Purified protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/4000
Secondary antibody
Dilution
1/8000
Name
Non-Abcam antibody was used: anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Non protease cleaved protein, only full size protein band detected. No band at predicted post-cleavage size.
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
Protease cleaved purified protein, size shift observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ya Zhou
Verified customer
提交于 Jun 28 2016