样品类型Cell culture supernatant
灵敏度< 0.6 ng/ml
范围0.68 ng/ml - 500 ng/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 105.9 93% - 115%
实验步骤Multiple steps standard assay
Abcam’s TIE1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE1 in cell culture supernatants.
This assay employs an antibody specific for Human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Tie-1 in normal serum with ab100649, only in serum spiked with Human Tie-1. Plasma is not recommended.
Serum has been validated as a sample matrix with this kit. However, TIE1 concentrations are low in normal serum and therefore may not be detected by this assay.
Plasma is not recommended.
Optimization may be required with urine samples.
经测试应用适用于: Sandwich ELISAmore details
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human TIE1 2 vials Recombinant Human TIE1 Standard (lyophilized) 2 vials Stop Solution 1 x 25ml TIE1 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml
相关性TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
- EC 22.214.171.124
- receptor tyrosine kinase
- SwissProt: P35590 Human
Our Abpromise guarantee covers the use of ab100649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100649 has not yet been referenced specifically in any publications.