概述

  • 产品名称
    人Survivin ELISA试剂盒
    参阅全部 Survivin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Jurkat cells 5 2.5%
    批次间
    样品 n Mean SD CV%
    Jurkat cells 3 5.5%
  • 样品类型
    Cell culture supernatant, Urine, Serum, Adherent cells, Suspension cells, Tissue Extracts, Citrate Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    7 pg/ml
  • 范围
    93.75 pg/ml - 6000 pg/ml
  • 回收率

    79.5 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 86.4 85.4% - 87.4%
    Urine 91.6 89.9% - 92.6%
    Serum 79.5 78.5% - 80.5%
    Citrate Plasma 99.2 94.6% - 102.3%

  • 检测时间
    1h 30m
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Survivin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit (ab183361) is designed for the quantitative measurement of Survivin protein in human cell culture supernatant, serum, plasma, urine, cell and tissue extract samples.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.



    Sensitivity:  
    Samples diluted in Sample Diluent NS - 28 pg/mL
    Samples diluted in Sample Diluent 75BP - 7 pg/mL
    Samples diluted in 1X Cell Extraction Buffer - 25 pg/mL

  • 说明

    Survivin is a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and it participates in the organization of the center spindle by associating with polymerized microtubules. Survivin complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Survivin may counteract a default induction of apoptosis in G2/M phase. The acetylated form of Survivin represses STAT3 transactivation of target gene promoters. Survivin may play a role in neoplasia. Survivin is an inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 of Survivin do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab183361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve comparison between human survivin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Figure 1. Example of Survivin standard curve for cell culture supernatant and urine samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

  • Figure 2. Example of Survivin standard curve for serum and plasma-citrate samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

  • Figure 3. Example of Survivin standard curve for cell and tissue extract samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

  • Figure 4. Titration of Jurkat cell extract within the working range of the assay. Background-subtracted data values (mean +/- SD, n=2) are graphed.

  • Figure 5. Survivin concentrations in 10 individual Human serum donors. Two fold diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in pg of Survivin per mL of serum (mean +/- SD, n=3).

  • Figure 6. Comparison of Survivin concentrations in media and MCF7 cell supernatants. MCF7 cells were grown in 10F HGDMEM medium and Survivin concentrations were measured in undiluted cell culture supernatant (SN) sample and the 10F HGDMEM medium using this kit. Interpolated data values are graphed (mean +/- SD, n=2). Note that no detectable Survivin concentrations in growth medium were observed.

  • Figure 7. Comparison of Survivin concentrations in control and nocodazole treated HeLa cells. HeLa cells were grown in 10F HGDMEM medium for 17 hours in the presence of 200 ng/mL nocodazole or drug’s vehicle (DMSO). Survivin concentrations were measured in cell extracts diluted to 125 and 62.5 µg/mL using this kit. Interpolated data values expressed in pg Survivin per g of cell extract are graphed (mean +/- SD, n=2).

实验方案

文献

This product has been referenced in:
  • Liu Y  et al. Identification of bile survivin and carbohydrate antigen 199 in distinguishing cholangiocarcinoma from benign obstructive jaundice. Biomark Med 11:11-18 (2017). Sandwich ELISA ; Human . Read more (PubMed: 27874286) »
See 1 Publication for this product

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