人Survivin ELISA试剂盒(ab119607)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Survivin ELISA试剂盒
参阅全部 Survivin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human Survivin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Survivin in cell culture supernatants, serum and plasma (heparin, EDTA).
A Survivin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Survivin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Survivin amount of sample captured in plate.
Get better reproducibility in only 90 minutes with Human Survivin ELISA Kit (ab183361) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Survivin antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Survivin antibody 1 x 100µl Lyophilized recombinant Human Survivin standard 2 x 4ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
组织特异性
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
序列相似性
Belongs to the IAP family.
Contains 1 BIR repeat. -
发展阶段
Expression is cell cycle-dependent and peaks at mitosis. -
结构域
The BIR repeat is necessary and sufficient for HBXIP binding. -
翻译后修饰
Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
细胞定位
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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别名
- API4
- Apoptosis inhibitor 4
- Apoptosis inhibitor survivin
see all -
数据库链接
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: O15392 Human
- Unigene: 514527 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119607 尚未被引用在任何文献中。