Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.55 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人Soluble TNF Receptor I ELISA试剂盒
参阅全部 TNF Receptor I 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 1 pg/ml
范围0.55 pg/ml - 400 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 80.02 71% - 90% Serum 80.43 72% - 91% Plasma 81.36 73% - 91%
实验步骤Multiple steps standard assay
Abcam’s Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human Soluble TNF Receptor I 2 vials Recombinant Human Soluble TNF Receptor I Standard (lyophilized) 2 vials Soluble TNF Receptor I Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
疾病相关Familial hibernian fever
Multiple sclerosis 5
序列相似性Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
结构域The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
翻译后修饰The soluble form is produced from the membrane form by proteolytic processing.
细胞定位Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Information by UniProt
ab100642 被引用在 3 文献中.
- Mortaz E et al. Increased Serum Levels of Soluble TNF-a Receptor Is Associated With ICU Mortality in COVID-19 Patients. Front Immunol 12:592727 (2021). PubMed: 33968010
- Hosic S et al. Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-a Induced Injury. Cell Mol Bioeng 13:487-505 (2020). PubMed: 33184579
- Strøm Halden TA et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). PubMed: 24452849