人SAA ELISA试剂盒(SAA1) (ab100635)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.41 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人SAA ELISA试剂盒(SAA1) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.5 ng/ml -
范围
0.41 ng/ml - 300 ng/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 93.75 86% - 102% Serum 114.8 105% - 124% Plasma 112.1 83% - 137% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human SAA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAA in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Serum Amyloid A coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum Amyloid A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Serum Amyloid A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Serum Amyloid A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples. ab100635 cross reacts with SAA2.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human SAA 2 vials Recombinant Human SAA Standard (lyophilized) 2 vials Serum Amyloid A Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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别名
- Amyloid fibril protein AA
- Amyloid protein A
- MGC111216
see all -
数据库链接
- SwissProt: P0DJI8 Human
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab100635 被引用在 13 文献中.
- Wu JL et al. Acute-phase serum amyloid A for early detection of hepatocellular carcinoma in cirrhotic patients with low AFP level. Sci Rep 12:5799 (2022). PubMed: 35388082
- Metwalli O et al. Study of Some Inflammatory Mediators in the Serum of Patients With Atherosclerosis and Acute Myocardial Infarction. Cureus 13:e18450 (2021). PubMed: 34745775
- Lu H et al. Association Between Serum Amyloid A Expression and Disease Control after Endoscopic Sinus Surgery in Patients With Chronic Rhinosinusitis With Nasal Polyps. Ear Nose Throat J N/A:1455613211051311 (2021). PubMed: 34814741
- Chang C et al. Serum amyloid A1 can be a novel biomarker for evaluating the presence and severity of acute coronary syndrome. Clin Biochem 85:27-32 (2020). PubMed: 32805223
- Ahn N & Kim K Can Active Aerobic Exercise Reduce the Risk of Cardiovascular Disease in Prehypertensive Elderly Women by Improving HDL Cholesterol and Inflammatory Markers? Int J Environ Res Public Health 17:N/A (2020). PubMed: 32824020