人SDF1 alpha + SDF1 beta ELISA试剂盒(ab100637)
Key features and details
- Sensitivity: 42 pg/ml
- Range: 42 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人SDF1 alpha + SDF1 beta ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 42 pg/ml -
范围
42 pg/ml - 6000 pg/ml -
回收率
> 80 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94.63 86% - 104% Plasma 89.34 85% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human SDF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100637) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human SDF-1 alpha in normal serum with ab100637, only in serum spiked with Human SDF-1 alpha.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human SDF1 alpha 2 vials Recombinant Human SDF1 alpha Standard (lyophilized) 2 vials SDF1 alpha Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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细胞定位
SDF1 beta: Secreted. -
数据库链接
- Entrez Gene: 6387 Human
- Omim: 600835 Human
- SwissProt: P48061 Human
- SwissProt: P48061 Human
- Unigene: 522891 Human
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab100637 被引用在 14 文献中.
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e54271 (2022). PubMed: 35403791
- Lu X et al. CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming. Anim Cells Syst (Seoul) 26:148-157 (2022). PubMed: 36046033
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e55871 (2022). PubMed: 36200589
- Liu L et al. Enhanced Effect of IL-1ß-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling. Stem Cells Int 2020:2803747 (2020). PubMed: 32377202
- Yang X et al. Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell. Medicine (Baltimore) 99:e20944 (2020). PubMed: 32629699