人S100A9抗体Pair - BSA and Azide free (ab241896)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
概述
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产品名称
人S100A9抗体Pair - BSA and Azide free
参阅全部 S100A9 试剂盒 -
检测类型
ELISA set -
范围
62.5 pg/ml - 4000 pg/ml -
种属反应性
与反应: Human -
产品概述
The Antibody Pair can be used to quantify Human S100A9. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent Matched Antibody Pair Kit (ab222271), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
无载体
是 -
组件 10 x 96 tests Human S100A9 Capture Antibody (unconjugated) 1 x 100µg Human S100A9 Detector Antibody (unconjugated) 1 x 100µg -
研究领域
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功能
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
组织特异性
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
翻译后修饰
Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
细胞定位
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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别名
- Leukocyte L1 complex heavy chain
- 60B8AG
- CAGB
see all -
数据库链接
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab241896于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
图片
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To learn more about the advantages of recombinant antibodies see here.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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