人PSA ELISA试剂盒(ab264615)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PSA ELISA试剂盒
参阅全部 Prostate Specific Antigen 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Urine 8 5.9% 批次间 样品 n Mean SD CV% Urine 3 8.4% -
样品类型
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
8 pg/ml -
范围
39 pg/ml - 2500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 92 86% - 97% Urine 116 112% - 120% Serum 99 95% - 103% Hep Plasma 86 85% - 88% EDTA Plasma 88 80% - 94% Cit plasma 115 108% - 120% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human PSA ELISA Kit (ab264615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSA protein in edta plasma, hep plasma, serum, fetal bovine serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSA with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland, but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels. This assay detects both total and free PSA levels.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human PSA Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Human PSA Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 10 x 6ml 1 x 6ml Human PSA Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seals 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 1 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究领域
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功能
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- antigen, prostate-specific
- APS
- Gamma seminoprotein
see all -
数据库链接
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
相关产品
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Alternative Versions
图片
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Interpolated concentration of native PSA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human PSA standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Standard Curve comparison between human PSA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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The PSA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 630 pg/mL in serum, 1,300 pg/mL in plasma (citrate).
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The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 174 ng/mL in male urine.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 826 pg/mL with a range of 234 – 2,201 pg/mL.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Female urine was below the lower limit of detection and 174 ng/mL in male urine.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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