概述

  • 产品名称
    人Progesterone ELISA试剂盒
    参阅全部 Progesterone 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Serum
  • 检测类型
    Competitive
  • 灵敏度
    = 0.05 ng/ml
  • 范围
    0.05 ng/ml - 50 ng/ml
  • 回收率

    111.3 %

    特定样本回收率
    样品类型 平均% 范围
    Serum 111.3 0.8ng/ml - 43.1ng/ml

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    ab108654 is intended for the quantitative determination of progesterone concentration in Human serum.


    Progesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. Cholesterol is transformed to pregnenolone which is then converted via a combined dehydrogenase and isomerase to progesterone. The principle production sites are the adrenals and ovaries and the placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by the kidneys.

  • 经测试应用
    适用于: ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests
    Goat anti-rabbit IgG Coated Microplate (12 x 8 wells) 1 unit
    Progesterone Control 1 1 x 0.5ml
    Progesterone Control 2 1 x 0.5ml
    Progesterone Reference Standard 1 (0 ng/mL) 1 x 0.5ml
    Progesterone Reference Standard 2 (0.5 ng/mL) 1 x 0.5ml
    Progesterone Reference Standard 3 (3.0 ng/mL) 1 x 0.5ml
    Progesterone Reference Standard 4 (10 ng/mL) 1 x 0.5ml
    Progesterone Reference Standard 5 (25 ng/mL) 1 x 0.5ml
    Progesterone Reference Standard 6 (50 ng/mL) 1 x 0.5ml
    Progesterone-HRP Conjugate Concentrate (11x) 1 x 1.3ml
    Progesterone-HRP Conjugate Diluent 1 x 13ml
    Rabbit Anti-Progesterone Reagent (0.01%) pink color 1 x 7ml
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

图片

  • Progesterone standard curve

实验方案

文献

This product has been referenced in:
  • Zheng R  et al. A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher-Neuhäuser syndrome. Mol Med Rep 18:261-267 (2018). Read more (PubMed: 29749493) »
See 1 Publication for this product

客户评价及客户问答

1-2 of 2 Q&A

Answer

Thank you for contacting Abcam regarding ab108654.


Regarding this Progesterone Human ELISA kit, it is only validated for human serum and we do not recommend using cell culture medium.


Each kit is provided with 1 96 well plate.


Both Progesterone Reference Standards (of known concentration) and Progesterone Controls are provided to ensure that the kit is working properly.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you very much for your inquiry.

We do have no ELISA kit against LH, FSH or Progesterone which are tested against the rat proteins. We do have however such kits tested against the human protein, for which we have however not yet tested whether they can work with the rat protein.

I am inquiring with the laboratory whether we have any anti rat ELISA kits for these proteins which we could put in ourcatalogue and I will get back to you as soon as I know more.

Thank you for your patience. Please let me know if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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