人PreAlbumin ELISA试剂盒(Transthyretin) (ab108895)
Key features and details
- Sensitivity: 75 pg/ml
- Range: 0.122 ng/ml - 31.25 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PreAlbumin ELISA试剂盒(Transthyretin)
参阅全部 Prealbumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.7% 批次间 样品 n Mean SD CV% Overall 9.4% -
样品类型
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 75 pg/ml -
范围
0.122 ng/ml - 31.25 ng/ml -
回收率
= 98 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human PreAlbumin ELISA kit ((Transthyretin) is designed for the quantitative measurement of PreAlbumin concentrations in plasma, serum, milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.
A PreAlbumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PreAlbumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PreAlbumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human PreAlbumin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml PreAlbumin Microplate (12 x 8 well strips) 1 unit PreAlbumin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. -
组织特异性
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. -
疾病相关
Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. -
序列相似性
Belongs to the transthyretin family. -
结构域
Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. -
细胞定位
Secreted. Cytoplasm. - Information by UniProt
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别名
- Amyloid polyneuropathy
- Amyloidosis I
- ATTR
see all -
数据库链接
- Entrez Gene: 7276 Human
- Omim: 176300 Human
- SwissProt: P02766 Human
- Unigene: 427202 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108895 被引用在 7 文献中.
- Carbonaro M et al. Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model. Sci Rep 12:14079 (2022). PubMed: 35982097
- Huang M et al. Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis. Front Neurol 13:886040 (2022). PubMed: 36003300
- Niemietz C et al. APOE polymorphism in ATTR amyloidosis patients treated with lipid nanoparticle siRNA. Amyloid 27:45-51 (2020). PubMed: 31648569
- Kan HW et al. Sensory nerve degeneration in a mouse model mimicking early manifestations of familial amyloid polyneuropathy due to transthyretin Ala97Ser. Neuropathol Appl Neurobiol 44:673-686 (2018). PubMed: 29423915
- Murakami Y et al. Spontaneous intracranial hypotension is diagnosed by a combination of lipocalin-type prostaglandin D synthase and brain-type transferrin in cerebrospinal fluid. Biochim Biophys Acta 1862:1835-1842 (2018). PubMed: 29621631
- Ambrosius W et al. Predictive value of serum transthyretin for outcome in acute ischemic stroke. PLoS One 12:e0179806 (2017). PubMed: 28636639
- Tong M et al. Aggregated transthyretin is specifically packaged into placental nano-vesicles in preeclampsia. Sci Rep 7:6694 (2017). PubMed: 28751735