人PD-1 ELISA试剂盒(ab252360)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.6 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PD-1 ELISA试剂盒
参阅全部 PD1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 2.4% 批次间 样品 n Mean SD CV% Serum 3 9.6% -
样品类型
Cell culture supernatant, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
9.6 pg/ml -
范围
25 pg/ml - 1600 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 93 90% - 96% Tissue Extracts 102 101% - 104% Cell Lysate 98 95% - 100% Cell culture media 95 93% - 97% Hep Plasma 92 90% - 94% EDTA Plasma 95 93% - 97% Cit plasma 94 93% - 96% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human PD-1 ELISA Kit (ab252360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1 protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-1 with 9.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 96 tests 10X Human PD-1 Capture Antibody 1 x 600µl 1 x 600µl 10X Human PD-1 Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 10ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human PD-1 Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究领域
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功能
Possible cell death inducer, in association with other factors. -
疾病相关
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
发展阶段
Induced at programmed cell death. -
细胞定位
Membrane. - Information by UniProt
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别名
- CD279
- CD279 antigen
- hPD 1
see all -
数据库链接
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of PD-1 were measured in duplicates, interpolated from the PD-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (EDTA) neat, plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 119.1 pg/mL in serum, 88.1 pg/mL in plasma (citrate) and 85.9 pg/mL in plasma (EDTA), and 92.8 pg/mL in plasma (heparin).
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The concentrations of PD-1 were measured in duplicate and interpolated from the PD-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 636.9 pg/mL in Molt-4 cell extract and 566.5 pg/mL in Thymus tissue extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 86.56 pg/mL with a range of 47.82 – 210.99 pg/mL.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab252360 被引用在 3 文献中.
- Huyen PTM et al. Circulating level of sPD-1 and PD-1 genetic variants are associated with hepatitis B infection and related liver disease progression. Int J Infect Dis 115:229-236 (2022). PubMed: 34910956
- El-Sayed WA et al. Novel 1,2,3-Triazole-Coumarin Hybrid Glycosides and Their Tetrazolyl Analogues: Design, Anticancer Evaluation and Molecular Docking Targeting EGFR, VEGFR-2 and CDK-2. Molecules 27:N/A (2022). PubMed: 35408446
- Pesini C et al. PD-1 is expressed in cytotoxic granules of NK cells and rapidly mobilized to the cell membrane following recognition of tumor cells. Oncoimmunology 11:2096359 (2022). PubMed: 35813574