产品名称抗人Papillomavirus 16 (E7)抗体[289-17013]
参阅全部 Human Papillomavirus 16 (E7) 一抗
描述小鼠单克隆抗体[289-17013] to人Papillomavirus 16 (E7)
经测试应用适用于: IP, WB, ELISA, RIA, Flow Cyt, IHC-Frmore details
种属反应性与反应: Other species
Full length protein corresponding to Human Papillomavirus 16 (E7). Immunogen: HPV 16 E7 synthesized in S. pombe.
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab20191 as a possible replacement.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Concentration information loading...
纯化说明Purified from ascites.
Our Abpromise guarantee covers the use of ab30731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.|
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
相关性E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
- E7 antibody
- HPV16 E7 antibody
- Protein E7 antibody
- Transforming protein antibody
This product has been referenced in:
- Liu Y et al. Nuclear lactate dehydrogenase A senses ROS to produce a-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun 9:4429 (2018). Read more (PubMed: 30356100) »
- Roopngam PE Increased Response of Human T-Lymphocytes by Dendritic Cells Pulsed with HPV16E7 and Pleurotus sajor-caju-ß-glucan (PBG). Iran J Immunol 15:246-255 (2018). Read more (PubMed: 30593739) »