人Osteoactivin ELISA试剂盒(ab193711)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 45 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Osteoactivin ELISA试剂盒
参阅全部 GPNMB 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
45 pg/ml -
范围
45 pg/ml - 12000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 117.8 111% - 126% Plasma 116.5 107% - 126% Cell culture media 112.9 98% - 149% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Osteoactivin Human ELISA Kit (ab193711) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoactivin in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Osteoactivin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Osteoactivin present in the samples. The wells are washed and biotinylated anti-Human Osteoactivin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Osteoactivin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human Osteoactivin detection antibody 2 vials Human Osteoactivin Standard (lyophilized) 2 vials Pre-coated Human Osteoactivin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Could be a melanogenic enzyme. -
组织特异性
Not restricted to the melanocytic lineage. -
序列相似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
发展阶段
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
细胞定位
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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别名
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
数据库链接
- Entrez Gene: 10457 Human
- Omim: 604368 Human
- SwissProt: Q14956 Human
- Unigene: 190495 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab193711 被引用在 2 文献中.
- Taghizadeh LA et al. Glycoprotein nonmetastatic melanoma protein B (GNMPB) as a novel biomarker for cerebral adrenoleukodystrophy. Sci Rep 12:7985 (2022). PubMed: 35568699
- Wang Q et al. GPNMB Extracellular Fragment Protects Melanocytes from Oxidative Stress by Inhibiting AKT Phosphorylation Independent of CD44. Int J Mol Sci 22:N/A (2021). PubMed: 34639184