抗人核Antigen抗体[235-1] (ab191181)
Key features and details
- Mouse monoclonal [235-1] to Human Nuclear Antigen
- Suitable for: Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Human, Non human primates
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
抗人核Antigen抗体[235-1]
参阅全部 Human Nuclear Antigen 一抗 -
描述
小鼠单克隆抗体[235-1] to人核Antigen -
宿主
Mouse -
特异性
ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations. -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Human, Non human primates
不与反应: Mouse, Rat, Chicken -
免疫原
Tissue, cells or virus corresponding to Human Human Nuclear Antigen. Nuclei of Human myeloid leukemia biopsy cells
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阳性对照
- IHC-Fr: Human tonsil tissue; ICC/IF: MCF and K562 cells; Flow Cyt: Raji, MCF7, K562, HeLa, and Jurkat cells.
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常规说明
ab190710 is recommended for IHC-P with paraffin embedded sections.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
235-1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191181于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
Use a concentration of 1 - 2 µg/ml.
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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说明 |
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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别名
- HNA antibody
- HuNu antibody
图片
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PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
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Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Characterization of LM-NSC008 cells.
Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
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Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 µg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
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PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
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Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (43)
ab191181 被引用在 43 文献中.
- Zhao Y et al. Human ESC-derived immunity- and matrix- regulatory cells ameliorated white matter damage and vascular cognitive impairment in rats subjected to chronic cerebral hypoperfusion. Cell Prolif 55:e13223 (2022). PubMed: 35437845
- Ikeda M et al. TERT/BMI1-transgenic human dermal papilla cells enhance murine hair follicle formation in vivo. J Dermatol Sci 106:78-85 (2022). PubMed: 35440400
- Chao J et al. Therapeutic development for Canavan disease using patient iPSCs introduced with the wild-type ASPA gene. iScience 25:104391 (2022). PubMed: 35637731
- Zhao P et al. LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Mol Neurodegener 17:44 (2022). PubMed: 35717259
- Gonzaga VF et al. Therapeutic Potential of Human Immature Dental Pulp Stem Cells Observed in Mouse Model for Acquired Aplastic Anemia. Cells 11:N/A (2022). PubMed: 35883695