人NOV ELISA试剂盒(Nephroblastoma Overexpressed) (ab193710)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人NOV ELISA试剂盒(Nephroblastoma Overexpressed)
参阅全部 CCN3 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
2 pg/ml -
范围
2 pg/ml - 30000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 109.5 102% - 118% Plasma 99.29 93% - 106% Cell culture media 130.4 118% - 139% -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Abcam’s NOV (Nephroblastoma Overexpressed) Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NOV in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures NOV present in the samples. The wells are washed and biotinylated anti-human NOV antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of NOV bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human NOV detection antibody 2 vials Human NOV Standards (lyophilized) 2 vials Pre-coated Human NOV Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Immediate-early protein likely to play a role in cell growth regulation. -
组织特异性
Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. -
序列相似性
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- CCN family member 3
- CCN3
- IBP-9
see all -
数据库链接
- Entrez Gene: 4856 Human
- Omim: 164958 Human
- SwissProt: P48745 Human
- Unigene: 235935 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab193710 被引用在 1 文献中.
- Li H et al. The Predictive Role of Tenascin-C and Cellular Communication Network Factor 3 (CCN3) in Post Hepatectomy Liver Failure in a Rat Model and 50 Patients Following Partial Hepatectomy. Med Sci Monit 25:6755-6766 (2019). PubMed: 31494663