人Neuron specific Enolase ELISA试剂盒(ab217778)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人Neuron specific Enolase ELISA试剂盒
参阅全部 NSE 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5 4.3% 批次间 样品 n Mean SD CV% Overall 3 11.4% -
样品类型
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
23 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 106 103% - 108% Cell culture extracts 90 89% - 91% Tissue Extracts 110 106% - 113% Cell culture media 103 102% - 105% Hep Plasma 109 104% - 114% EDTA Plasma 105 96% - 118% Cit plasma 102 100% - 105% Cerebral Spinal Fluid 107 103% - 110% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Neuron specific Enolase ELISA Kit (ab217778) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuron specific Enolase protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuron specific Enolase with 23 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Neuron-specific Enolase (also known as NSE, gamma-enolase and Enolase 2) is a cytoplasmic phosphopyruvate hydratase. Neuron-specific Enolase has two related family members, Enolase 1 and Enolase 3. Neuron-specific Enolase can be used to identify neuronal cells and normal or malignant cells with neuroendocrine origin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 96 tests 10X Human Neuron-specific Enolase Capture Antibody 1 x 600µl 1 x 600µl 10X Human Neuron-specific Enolase Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 10ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human Neuron-specific Enolase Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究领域
-
功能
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
组织特异性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
序列相似性
Belongs to the enolase family. -
发展阶段
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
细胞定位
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
-
别名
- 2 phospho D glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- Eno 2
see all -
数据库链接
- Entrez Gene: 2026 Human
- Omim: 131360 Human
- SwissProt: P09104 Human
- Unigene: 511915 Human
相关产品
-
Alternative Versions
图片
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Standard curve comparison between human Neuron specific Enolase SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 821 pg/mL in serum, 8545 pg/mL in plasma (EDTA), 1194 pg/mL in plasma (heparin), and 3386 pg/mL in plasma (citrate).
-
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Eight out of ten donors tested below the detectable dose. The mean Neuron-specific Enolase concentration of two donors was determined to be 1553 pg/mL with a range of 766 – 2340 pg/mL.
-
PBMCs were grown in the absence (unstimulated) or presence (stimulated) of phytohemagllutinin (PHA) for 3 days. The concentrations of Neuron-specific Enolase were measured in duplicates, interpolated from the Neuron-specific Enolase standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50%, and stimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be undetectable in media, 711 pg/mL in unstimulated, and 4051 pg/mL in stimulated.
-
The concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 5828 pg/mL in SH-SY5Y cell extract and 1482 pg/mL in human brain homogenate tissue extract.
-
To learn more about the advantages of recombinant antibodies see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab217778 被引用在 3 文献中.
- Jansen van Rensburg M et al. Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation. Stem Cells Int 2022:2744789 (2022). PubMed: 36106176
- Li J et al. Immortalized Human Bone Marrow Derived Stromal Cells in Treatment of Transient Cerebral Ischemia in Rats. J Alzheimers Dis 69:871-880 (2019). PubMed: 31156178
- Zhu J et al. Effects of neuritin on the differentiation of bone marrow-derived mesenchymal stem cells into neuron-like cells. Mol Med Rep 16:3201-3207 (2017). PubMed: 28714031