人Neurogranin ELISA试剂盒(NRGN) (ab277083)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Neurogranin ELISA试剂盒(NRGN)
参阅全部 Neurogranin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Plasma 8 7.2% 批次间 样品 n Mean SD CV% Plasma 3 14.9% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.4 pg/ml -
范围
9.375 pg/ml - 600 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 76 72% - 78% Serum 86 80% - 93% EDTA Plasma 96 88% - 103% Cit plasma 104 98% - 109% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Neurogranin ELISA kit (ab277083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurogranin protein in human serum, plasma - citrate, plasma EDTA, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurogranin with 1.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Neurogranin (NRGN), encoded by the NRGN gene, is a postsynaptic protein kinase that is highly expressed in the brain and regulates the availability of calmodulin; neurogranin binds to calmodulin in the absence of calcium. Phosphorylation by protein kinase C has been observed to decrease the calmodulin binding activity. Studies have previously shown that NRGN expression is regulated during development by thyroid hormones and retinoids for proper development of the central nervous system. ab277083 has been developed to specifically recognize full length neurogranin.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Neurogranin (NRGN) Capture Antibody 1 x 600µl 10X Human Neurogranin (NRGN) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Neurogranin (NRGN) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. -
组织特异性
In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease. -
序列相似性
Belongs to the neurogranin family.
Contains 1 collagen-like domain.
Contains 1 IQ domain. - Information by UniProt
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别名
- Protein kinase C substrate RC3
- Calmodulin binding protein
- Hng
see all -
数据库链接
- Entrez Gene: 4900 Human
- Omim: 602350 Human
- SwissProt: Q92686 Human
- Unigene: 524116 Human
相关产品
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Alternative Versions
图片
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The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Neurogranin were measured in duplicates, interpolated from the Neurogranin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurogranin concentration was determined to be 77 pg/mL in plasma (citrate).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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