Key features and details
- Sensitivity: 35 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
参阅全部 NBL1 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 35 pg/ml
范围27.43 pg/ml - 20000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 111.3 107% - 122% Serum 100.1 91% - 109% Plasma 81.82 72% - 90%
实验步骤Multiple steps standard assay
Abcam’s NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm..
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human NBL1 2 vials NBL1 Microplate (12 strips x 8 wells) 1 unit Recombinant Human NBL1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Information by UniProt
- DAN domain family member 1